ArticleBMC molecular biology2008
Analysis of chromatin boundary activity in Drosophila cells.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.Nucleic acids research · 2022Article
- A Cell Density-Dependent Reporter in the Drosophila S2 Cells.Scientific reports · 2019Article
- Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements.Nucleic acids research · 2016Article
- Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family.Genes & development · 2015Article
- dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes.Nucleic acids research · 2014Article
- Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae.Nucleic acids research · 2013Article
- Chromatin domain boundary element search tool for Drosophila.Nucleic acids research · 2012Article
- Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster.Molecular and cellular biology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChromatin boundaries, also known as insulators, regulate gene activity by organizing active and repressive chromatin domains and modulate enhancer-promoter interactions. However, the mechanisms of boundary action are poorly understood, in part due to our limited knowledge about insulator proteins, and a shortage of standard assays by which diverse boundaries could be compared.
resultsWe report here the development of an enhancer-blocking assay for studying insulator activity in Drosophila cultured cells. We show that the activities of diverse Drosophila insulators including suHw, SF1, SF1b, Fab7 and Fab8 are supported in these cells. We further show that double stranded RNA (dsRNA)-mediated knockdown of SuHw and dCTCF factors disrupts the enhancer-blocking function of suHw and Fab8, respectively, thereby establishing the effectiveness of using RNA interference in our cell-based assay for probing insulator function.
conclusionThe novel boundary assay provides a quantitative and efficient method for analyzing insulator mechanism and can be further exploited in genome-wide RNAi screens for insulator components. It provides a useful tool that complements the transgenic and genetic approaches for studying this important class of regulatory elements.
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