ArticleInfection and immunity2009
Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 124 citations in OpenAlex.
- Characterization of Host Protein Prohibitin-2 Interacting With Cryptosporidium parvum Cyclophilin 23.Transboundary and emerging diseases · 2026Article
- LncRNA BACE1-AS delays the propagation ofMicrobiology spectrum · 2025Article
- Deciphering Host-Pathogen Interactions: Role ofPathogens (Basel, Switzerland) · 2025Review
- Article
- Fighting eimeriosis by using the anti-eimerial and anti-apoptotic properties of rhatany root extract.Frontiers in immunology · 2024Article
- Gene expression profile of HCT-8 cells following single or co-infections with Cryptosporidium parvum and bovine coronavirus.Scientific reports · 2023Article
- A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4BMC veterinary research · 2023Article
- MiR-3976 regulates HCT-8 cell apoptosis and parasite burden by targeting BCL2A1 in response to Cryptosporidium parvum infection.Parasites & vectors · 2023Article
- Cryptosporidium parvumcompetes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves.Veterinary research · 2023Article
- Role of Cryptosporidium spp in Development of Colorectal Cancer.Asian Pacific journal of cancer prevention : APJCP · 2023Article
- Artificial Infestation ofInternational journal of molecular sciences · 2023Article
- The role of atypical MAP kinase 4 in the host interaction with Cryptosporidium parvum.Scientific reports · 2023Article
- Early immune and host cell responses toFrontiers in parasitology · 2023Article
- Apoptotic changes in the intestinal epithelium ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Whole transcriptome analysis of HCT-8 cells infected by Cryptosporidium parvum.Parasites & vectors · 2022Article
- MiR-942-5p targeting the IFI27 gene regulates HCT-8 cell apoptosis via a TRAIL-dependent pathway during the early phase of Cryptosporidium parvum infection.Parasites & vectors · 2022Article
- IsFood and waterborne parasitology · 2022Article
- Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB signaling pathway.Parasites & vectors · 2021Article
- Cryptosporidial Infection Suppresses Intestinal Epithelial Cell MAPK Signaling Impairing Host Anti-Parasitic Defense.Microorganisms · 2021Article
- The Mucosal Innate Immune Response toFrontiers in cellular and infection microbiology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The impact of Cryptosporidium parvum infection on host cell gene expression was investigated by microarray analysis with an in vitro model using human ileocecal HCT-8 adenocarcinoma cells. We found changes in 333 (2.6%) transcripts at at least two of the five (6, 12, 24, 48, and 72 h) postinfection time points. Fifty-one of the regulated genes were associated with apoptosis and were grouped into five clusters based on their expression patterns. Early in infection (6 and 12 h), genes with antiapoptotic roles were upregulated and genes with apoptotic roles were downregulated. Later in infection (24, 48, and 72 h), proapoptotic genes were induced and antiapoptotic genes were downregulated, suggesting a biphasic regulation of apoptosis: antiapoptotic state early and moderately proapoptotic state late in infection. This transcriptional profile matched the actual occurrence of apoptosis in the infected cultures. Apoptosis was first detected at 12 h postinfection and increased to a plateau at 24 h, when 20% of infected cells showed nuclear condensation. In contrast, experimental silencing of Bcl-2 induced apoptosis in 50% of infected cells at 12 h postinfection. This resulted in a decrease in the infection rate and a reduction in the accumulation of meront-containing cells. To test the significance of the moderately proapoptotic state late in the infection, we inhibited apoptosis using pancaspase inhibitor Z-VAD-FMK. This treatment also affected the progression of C. parvum infection, as reinfection, normally seen late (24 h to 48 h), did not occur and accumulation of mature meronts was impaired. Control of host apoptosis is complex and crucial to the life of C. parvum. Apoptosis control has at least two components, early inhibition and late moderate promotion. For a successful infection, both aspects appear to be required.
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Registered trials
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