ArticleMolecular and cellular biology2009
The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Genomically integrated cassettes swapping: bringing modularity to the strain level in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- TORC2 inhibition triggers yeast chromosome fragmentation through misregulated Base Excision Repair of clustered oxidation events.Nature communications · 2024Article
- Human ALKBH6 Is Required for Maintenance of Genomic Stability and Promoting Cell Survival During Exposure of Alkylating Agents in Pancreatic Cancer.Frontiers in genetics · 2021Article
- Repair of base damage within break-induced replication intermediates promotes kataegis associated with chromosome rearrangements.Nucleic acids research · 2019Article
- Article
- Electrical discharges in water induce spores' DNA damage.PloS one · 2018Article
- Effective gene editing by high-fidelity base editor 2 in mouse zygotes.Protein & cell · 2017Article
- Mechanisms of glycosylase induced genomic instability.PloS one · 2017Article
- When DNA repair goes wrong: BER-generated DNA-protein crosslinks to oxidative lesions.DNA repair · 2016Review
- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Homologous recombination rescues ssDNA gaps generated by nucleotide excision repair and reduced translesion DNA synthesis in yeast G2 cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The preference for error-free or error-prone postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent.Molecular and cellular biology · 2013Article
- FOXP3 regulates sensitivity of cancer cells to irradiation by transcriptional repression of BRCA1.Cancer research · 2013Article
- Understanding the origins of UV-induced recombination through manipulation of sister chromatid cohesion.Cell cycle (Georgetown, Tex.) · 2012Article
- Differential effects of poly(ADP-ribose) polymerase inhibition on DNA break repair in human cells are revealed with Epstein-Barr virus.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Evidence for abasic site sugar phosphate-mediated cytotoxicity in alkylating agent treated Saccharomyces cerevisiae.PloS one · 2012Article
- Alkylation base damage is converted into repairable double-strand breaks and complex intermediates in G2 cells lacking AP endonuclease.PLoS genetics · 2011Article
- Damage-induced localized hypermutability.Cell cycle (Georgetown, Tex.) · 2011Article
- Characterizing resection at random and unique chromosome double-strand breaks and telomere ends.Methods in molecular biology (Clifton, N.J.) · 2011Article
- A single-strand specific lesion drives MMS-induced hyper-mutability at a double-strand break in yeast.DNA repair · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
DNA double-strand breaks can result from closely opposed breaks induced directly in complementary strands. Alternatively, double-strand breaks could be generated during repair of clustered damage, where the repair of closely opposed lesions has to be well coordinated. Using single and multiple mutants of Saccharomyces cerevisiae (budding yeast) that impede the interaction of DNA polymerase delta and the 5'-flap endonuclease Rad27/Fen1 with the PCNA sliding clamp, we show that the lack of coordination between these components during long-patch base excision repair of alkylation damage can result in many double-strand breaks within the chromosomes of nondividing haploid cells. This contrasts with the efficient repair of nonclustered methyl methanesulfonate-induced lesions, as measured by quantitative PCR and S1 nuclease cleavage of single-strand break sites. We conclude that closely opposed single-strand lesions are a unique threat to the genome and that repair of closely opposed strand damage requires greater spatial and temporal coordination between the participating proteins than does widely spaced damage in order to prevent the development of double-strand breaks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.