ArticleApplied and environmental microbiology2009
TatABC overexpression improves Corynebacterium glutamicum Tat-dependent protein secretion.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 68 citations in OpenAlex.
- Metabolic engineering strategies for sustainable de novo isobutylamine production by Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2026Article
- Purified protein glutaminase fromFood chemistry: X · 2024Article
- Exploring the secretome ofFrontiers in bioengineering and biotechnology · 2024Article
- Identification of multiple regulatory genes involved in TGase production inEngineering microbiology · 2023Article
- L-Glutamine-, peptidyl- and protein-glutaminases: structural features and applications in the food industry.World journal of microbiology & biotechnology · 2022Review
- Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process.Applied microbiology and biotechnology · 2022Article
- Enhanced production of neoagarobiose from agar with Corynebacterium glutamicum producing exo-type and endo-type β-agarases.Microbial biotechnology · 2021Article
- Droplet-Based Microfluidic High Throughput Screening ofFrontiers in bioengineering and biotechnology · 2021Article
- L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources.AMB Express · 2014Article
- Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum.Microbial cell factories · 2014Article
- Development of fatty acid-producing Corynebacterium glutamicum strains.Applied and environmental microbiology · 2013Article
- Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans.Microbial cell factories · 2013Article
- Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum.Microbial biotechnology · 2013Article
- Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum.Microbial cell factories · 2012Article
- The NMR structure of protein-glutaminase from Chryseobacterium proteolyticum.Journal of biomolecular NMR · 2010Article
- How to achieve high-level expression of microbial enzymes: strategies and perspectives.BioengineeredReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The twin-arginine translocation (Tat) pathway in Corynebacterium glutamicum has been described previously. The minimal functional Tat system in C. glutamicum required TatA and TatC but did not require TatB, although this component was required for maximal efficiency of Tat-dependent secretion. We previously demonstrated that Chryseobacterium proteolyticum pro-protein glutaminase (pro-PG) and Streptomyces mobaraensis pro-transglutaminase (pro-TG) could be secreted via the Tat pathway in C. glutamicum. Here we report that the amounts of pro-PG secreted were more than threefold larger when TatC or TatAC was overexpressed, and there was a further threefold increase when TatABC was overexpressed. These results show that the amount of TatC protein is the first bottleneck and the amount of TatB protein is the second bottleneck in Tat-dependent protein secretion in C. glutamicum. In addition, the amount of pro-TG that accumulated via the Tat pathway when TatABC was overexpressed with the TorA signal peptide in C. glutamicum was larger than the amount that accumulated via the Sec pathway. We concluded that TatABC overexpression improves Tat-dependent pro-PG and pro-TG secretion in C. glutamicum.
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