ArticleNucleic acids research2009
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 274 papers.
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Who cites it
274 citing papers in PubMed.
- Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects.Synthetic and systems biotechnology · 2026Review
- De novo coassembly of functional chlorophyll- and carotenoid-protein complexes inScience advances · 2026Article
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- De novo L-(+)-tartaric acid biosynthesis in multi-modular engineered yeasts.Nature communications · 2026Article
- An advanced genome engineering platform for Komagataella phaffii enabling versatile in vivo DNA assembly and multiplex integration.Journal of biological engineering · 2026Article
- Protocol for connector-enabled multigene pathway assembly using Golden Gate Assembly and yeast recombination.STAR protocols · 2026Article
- Enhancing delta-tocotrienol production inSynthetic and systems biotechnology · 2026Article
- PYEAST - A Computational Toolkit for Saccharomyces cerevisiae Genetic Engineering.NPJ systems biology and applications · 2026Article
- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Biological parts in yeast synthetic biology: from regulatory elements to predictive design platforms.FEMS yeast research · 2026Review
- Advanced applications of synthetic biology technology in biosynthesis of bioactive compounds from medicinal plants.Chinese herbal medicines · 2026Review
- Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast.Science advances · 2025Article
- In Vivo DNA Assembly inACS synthetic biology · 2025Article
- Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis.Nature communications · 2025Article
- Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder.Nature communications · 2025Article
- Engineering Baker's Yeast for Efficient cAMP Synthesis via Regulation of PKA Activity.Foods (Basel, Switzerland) · 2025Article
- Evolutionary engineering ofScience advances · 2024Article
- Rational construction of genome-minimizedSynthetic and systems biotechnology · 2024Article
- Establishing cell suitability for high-level production of licorice triterpenoids in yeast.Acta pharmaceutica Sinica. B · 2024Article
- Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
214 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional D-xylose utilization pathway (approximately 9 kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (approximately 11 kb DNA consisting of five genes) and a functional combined D-xylose utilization and zeaxanthin biosynthesis pathway (approximately 19 kb consisting of eight genes) with high efficiencies (70-100%) either on a plasmid or on a yeast chromosome. As this new method only requires simple DNA preparation and one-step yeast transformation, it represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.
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