ArticleJournal of bacteriology2009
Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum sensing.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- The role of bacterial signaling networks in antibiotics response and resistance regulation.Marine life science & technology · 2022Review
- Lactate-Induced Dispersal ofInfection and immunity · 2021Article
- The Role ofInfection and immunity · 2021Article
- Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance.Antimicrobial agents and chemotherapy · 2019Review
- Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae.Scientific reports · 2019Article
- Quorum sensing in thermophiles: prevalence of autoinducer-2 system.BMC microbiology · 2018Article
- Interference With Quorum-Sensing Signal Biosynthesis as a Promising Therapeutic Strategy Against Multidrug-Resistant Pathogens.Frontiers in cellular and infection microbiology · 2018Review
- Regulation of bacteria population behaviors by AI-2 "consumer cells" and "supplier cells".BMC microbiology · 2017Article
- The fitness burden imposed by synthesising quorum sensing signals.Scientific reports · 2016Article
- The Impact of Non-Enzymatic Reactions and Enzyme Promiscuity on Cellular Metabolism during (Oxidative) Stress Conditions.Biomolecules · 2015Review
- Pfs promotes autolysis-dependent release of eDNA and biofilm formation in Staphylococcus aureus.Medical microbiology and immunology · 2015Article
- Catalytic site cooperativity in dimeric methylthioadenosine nucleosidase.Biochemistry · 2014Article
- Sinefungin, a natural nucleoside analogue of S-adenosylmethionine, inhibits Streptococcus pneumoniae biofilm growth.BioMed research international · 2014Article
- A chemical biology approach to interrogate quorum-sensing regulated behaviors at the molecular and cellular level.Chemistry & biology · 2013Article
- Functional definition of LuxS, an autoinducer-2 (AI-2) synthase and its role in full virulence of Streptococcus suis serotype 2.Journal of microbiology (Seoul, Korea) · 2011Article
- Identification of a key amino acid of LuxS involved in AI-2 production in Campylobacter jejuni.PloS one · 2011Article
- Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.Molecular microbiology · 2011Review
- Enzyme-ligand interactions that drive active site rearrangements in the Helicobacter pylori 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase.Protein science : a publication of the Protein Society · 2010Article
- What's in a name? The semantics of quorum sensing.Trends in microbiology · 2010Article
- In Helicobacter pylori, LuxS is a key enzyme in cysteine provision through a reverse transsulfuration pathway.Journal of bacteriology · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The activated methyl cycle (AMC) is a central metabolic pathway used to generate (and recycle) several important metabolites and enable methylation. Pfs and LuxS are considered integral components of this pathway because they convert S-adenosylhomocysteine (SAH) to S-ribosylhomocysteine (SRH) and S-ribosylhomocysteine to homocysteine (HCY), respectively. The latter reaction has a second function since it also generates the precursor of the quorum-sensing molecule autoinducer 2 (AI-2). By demonstrating that there was a complete lack of AI-2 production in pfs mutants of the causative agent of meningitis and septicemia, Neisseria meningitidis, we showed that the Pfs reaction is the sole intracellular source of the AI-2 signal. Analysis of lacZ reporters and real-time PCR experiments indicated that pfs is expressed constitutively from a promoter immediately upstream, and careful study of the pfs mutants revealed a growth defect that could not be attributed to a lack of AI-2. Metabolite profiling of the wild type and of a pfs mutant under various growth conditions revealed changes in the concentrations of several AMC metabolites, particularly SRH and SAH and under some conditions also HCY. Similar studies established that an N. meningitidis luxS mutant also has metabolite pool changes and growth defects in line with the function of LuxS downstream of Pfs in the AMC. Thus, the observed growth defect of N. meningitidis pfs and luxS mutants is not due to quorum sensing but is probably due to metabolic imbalance and, in the case of pfs inactivation, is most likely due to toxic accumulation of SAH.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.