ArticleJournal of bacteriology2009
Roles of ExoI and SbcCD nucleases in "reckless" DNA degradation in recA mutants of Escherichia coli.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- In Vitro Antimicrobial Efficacy Assessment of Ethanolic, Aqueous, and Dual Solvent Extracts of MushroomAntibiotics (Basel, Switzerland) · 2024Article
- RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease.Microbiology and molecular biology reviews : MMBR · 2023Review
- Thymine-starvation-induced chromosomal fragmentation is not required for thymineless death in Escherichia coli.Molecular microbiology · 2022Article
- Competitive advantage of oxygen-tolerant bioanodes ofBiofilm · 2021Article
- Article
- The Roles of Bacterial DNA Double-Strand Break Repair Proteins in Chromosomal DNA Replication.FEMS microbiology reviews · 2020Review
- Sources of thymidine and analogs fueling futile damage-repair cycles and ss-gap accumulation during thymine starvation in Escherichia coli.DNA repair · 2019Article
- Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome.PLoS genetics · 2018Article
- 3'-Terminated Overhangs Regulate DNA Double-Strand Break Processing inG3 (Bethesda, Md.) · 2017Article
- Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB.PLoS genetics · 2017Article
- Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII.Journal of microbiology (Seoul, Korea) · 2015Article
- RecBCD is required to complete chromosomal replication: Implications for double-strand break frequencies and repair mechanisms.DNA repair · 2015Review
- Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.PLoS biology · 2014Article
- Isolation and validation of an endogenous fluorescent nucleoid reporter in Salmonella Typhimurium.PloS one · 2014Article
- ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complex.Nucleic acids research · 2012Article
- The DNA Exonucleases of Escherichia coli.EcoSal Plus · 2011Article
- The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair.Cell · 2011Article
- RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli.Genetics · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exponentially growing recA mutant cells of Escherichia coli display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence of this "reckless" DNA degradation, populations of recA mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate-cell production are efficiently suppressed by mutations in the xonA (sbcB) and sbcD genes. The suppressive effects of these mutations were observed in normally grown, as well as in UV-irradiated, recA cells. The products of the xonA and sbcD genes are known to code for the ExoI and SbcCD nucleases, respectively. Since both xonA and sbcD mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in recA cells. We suggest that their roles might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.