ArticleProceedings of the National Academy of Sciences of the United States of America2008
Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 74 citations in OpenAlex.
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- Extracting anomalous diffusion parameters from multi-state ensembles of short single molecule trajectories.bioRxiv : the preprint server for biology · 2026Article
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain.bioRxiv : the preprint server for biology · 2026Article
- Imaging Organization of RNA Processing within the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Structure and Dynamics of Mono- vs. Doubly Lipidated Rab5 in Membranes.International journal of molecular sciences · 2019Article
- The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms.The Journal of antimicrobial chemotherapy · 2019Article
- A Jump-Distance-Based Parameter Inference Scheme for Particulate Trajectories.Biophysical journal · 2019Article
- Nuclear actin: ancient clue to evolution in eukaryotes?Histochemistry and cell biology · 2018Review
- Diatrack particle tracking software: Review of applications and performance evaluation.Traffic (Copenhagen, Denmark) · 2017Review
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Monte Carlo simulations of protein micropatterning in biomembranes: effects of immobile sticky obstacles.Journal of physics D: Applied physics · 2016Article
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking.Nucleic acids research · 2015Article
- Cytoplasmic HIV-1 RNA is mainly transported by diffusion in the presence or absence of Gag protein.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Transcription regulation during stable elongation by a reversible halt of RNA polymerase II.Molecular biology of the cell · 2014Article
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- mRNA on the move: the road to its biological destiny.The Journal of biological chemistry · 2013Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Messenger ribonucleoprotein particles (mRNPs) move randomly within nucleoplasm before they exit from the nucleus. To further understand mRNP trafficking, we have studied the intranuclear movement of a specific mRNP, the BR2 mRNP, in salivary gland cells in Chironomus tentans. Their polytene nuclei harbor giant chromosomes separated by vast regions of nucleoplasm, which allows us to study mRNP mobility without interference of chromatin. The particles were fluorescently labeled with microinjected oligonucleotides (DNA or RNA) complementary to BR2 mRNA or with the RNA-binding protein hrp36, the C. tentans homologue of hnRNP A1. Using high-speed laser microscopy, we followed the intranuclear trajectories of single mRNPs and characterized their motion within the nucleoplasm. The Balbiani ring (BR) mRNPs moved randomly, but unexpectedly, in a discontinuous manner. When mobile, they diffused with a diffusion coefficient corresponding to their size. Between mobile phases, the mRNPs were slowed down 10- to 250-fold but were never completely immobile. Earlier electron microscopy work has indicated that BR particles can attach to thin nonchromatin fibers, which are sometimes connected to discrete fibrogranular clusters. We propose that the observed discontinuous movement reflects transient interactions between freely diffusing BR particles and these submicroscopic structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.