ArticleThe Journal of biological chemistry2009
Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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Who cites it
46 citing papers in PubMed, 68 citations in OpenAlex.
- Q586B2 is a crucial virulence factor during the early stages of Trypanosoma brucei infection that is conserved amongst trypanosomatids.Nature communications · 2024Article
- Structure, function and druggability of the African trypanosome flagellum.Journal of cellular physiology · 2022Review
- Structure of the trypanosome paraflagellar rod and insights into non-planar motility of eukaryotic cells.Cell discovery · 2021Article
- APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei.mSphere · 2021Article
- Trypanin Disruption Affects the Motility and Infectivity of the ProtozoanFrontiers in cellular and infection microbiology · 2021Article
- The single flagellum ofJournal of cell science · 2020Article
- Control of assembly of extra-axonemal structures: the paraflagellar rod of trypanosomes.Journal of cell science · 2020Article
- Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections.PLoS pathogens · 2019Article
- Cellular landmarks of Trypanosoma brucei and Leishmania mexicana.Molecular and biochemical parasitology · 2019Article
- Review
- DRC2/CCDC65 is a central hub for assembly of the nexin-dynein regulatory complex and other regulators of ciliary and flagellar motility.Molecular biology of the cell · 2018Article
- Novel Effects of Lapatinib Revealed in the African Trypanosome by Using Hypothesis-Generating Proteomics and Chemical Biology Strategies.Antimicrobial agents and chemotherapy · 2017Article
- Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation.Scientific reports · 2016Article
- Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum.Nature communications · 2015Article
- An overview on kinetoplastid paraflagellar rod.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Review
- Assembly and maintenance of the flagellum attachment zone filament in Trypanosoma brucei.Journal of cell science · 2015Article
- A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes.Journal of cell science · 2015Article
- Calcium signaling in trypanosomatid parasites.Cell calcium · 2015Review
- Motility and more: the flagellum of Trypanosoma brucei.Nature reviews. Microbiology · 2014Review
- Proteomic analysis of intact flagella of procyclic Trypanosoma brucei cells identifies novel flagellar proteins with unique sub-localization and dynamics.Molecular & cellular proteomics : MCP · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal protein components of flagellar structures via ablation by inducible RNA interference mutation. By this approach we identified 20 novel components of the paraflagellar rod (PFR). Using epitope tagging we validated a subset of these as being present within the PFR by immunofluorescence. Bioinformatic analysis of the PFR cohort reveals a likely calcium/calmodulin regulatory/signaling linkage between some components. We extended the RNA interference mutant/comparative proteomic analysis to individual novel components of our PFR proteome, showing that the approach has the power to reveal dependences between subgroups within the cohort.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.