ArticleJournal of experimental botany2009
Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.).
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Fruit-Specific Promoters in Plants: Advances, Regulatory Mechanisms and Applications in Plant Biotechnology.Plants (Basel, Switzerland) · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Integrated analysis of biparental and natural populations reveals CRIB domain-containing protein underlying seed coat crack trait in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Characterization of Transcription Regulatory Domains of OsMADS29: Identification of Proximal Auxin-Responsive Domains and a Strong Distal Negative Element.Frontiers in plant science · 2022Article
- Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology.BMC biotechnology · 2020Article
- Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.Molecular genetics and genomics : MGG · 2017Article
- Synthetic promoters in planta.Planta · 2015Review
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- Characterization of a maize Wip1 promoter in transgenic plants.International journal of molecular sciences · 2013Article
- Article
- Genome-wide characterization of phenylalanine ammonia-lyase gene family in watermelon (Citrullus lanatus).Planta · 2013Article
- Regulation of the fruit-specific PEP carboxylase SlPPC2 promoter at early stages of tomato fruit development.PloS one · 2012Article
- Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato.Plant cell reports · 2011Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 1.8 kb 5'-flanking region of the large subunit of ADP-glucose pyrophosphorylase, isolated from watermelon (Citrullus vulgaris S.), has fruit-specific promoter activity in transgenic tomato plants. Two negative regulatory regions, from -986 to -959 and from -472 to -424, were identified in this promoter region by fine deletion analyses. Removal of both regions led to constitutive expression in epidermal cells. Gain-of-function experiments showed that these two regions were sufficient to inhibit RFP (red fluorescent protein) expression in transformed epidermal cells when fused to the cauliflower mosaic virus (CaMV) 35S minimal promoter. Gel mobility shift experiments demonstrated the presence of leaf nuclear factors that interact with these two elements. A TCCAAAA motif was identified in these two regions, as well as one in the reverse orientation, which was confirmed to be a novel specific cis-element. A quantitative beta-glucuronidase (GUS) activity assay of stable transgenic tomato plants showed that the activities of chimeric promoters harbouring only one of the two cis-elements, or both, were approximately 10-fold higher in fruits than in leaves. These data confirm that the TCCAAAA motif functions as a fruit-specific element by inhibiting gene expression in leaves.
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