ArticleAmerican journal of physiology. Cell physiology2009
Characterization of putative stem cells in isolated human colonic crypt epithelial cells and their interactions with myofibroblasts.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Cross-kingdom dialogs in the gut: Integrating bacterial pathogens, helminths, and microbiota interactions for immune homeostasis.PLoS pathogens · 2025Review
- Analysis of mutations in tumor and normal adjacent tissue via fluorescence detection.Environmental and molecular mutagenesis · 2021Article
- Toll-like receptor expression in crypt epithelial cells, putative stem cells and intestinal myofibroblasts isolated from controls and patients with inflammatory bowel disease.Clinical and experimental immunology · 2014Article
- Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo.PLoS genetics · 2014Article
- Modeling of stem cell dynamics in human colonic crypts in silico.Journal of gastroenterology · 2014Article
- Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.World journal of gastroenterology · 2013Article
- Epimorphin deletion inhibits polyposis in the Apcmin/+ mouse model of colon carcinogenesis via decreased myofibroblast HGF secretion.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- A review of spatial computational models for multi-cellular systems, with regard to intestinal crypts and colorectal cancer development.Journal of mathematical biology · 2013Review
- Snail1 expression in colorectal cancer and its correlation with clinical and pathological parameters.BMC cancer · 2013Article
- The temporal and spatial expression patterns of ABCG2 in the developing human heart.International journal of cardiology · 2012Article
- Endocrine differentiation of rat enterocytes in long-term three-dimensional co-culture with intestinal myofibroblasts.In vitro cellular & developmental biology. Animal · 2011Article
- Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter.BMC cell biology · 2011Article
- A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells.Tissue engineering. Part C, Methods · 2011Article
- Intestinal subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium.PloS one · 2011Article
- Altered expression of a putative progenitor cell marker DCAMKL1 in the rat gastric mucosa in regeneration, metaplasia and dysplasia.BMC gastroenterology · 2010Article
- The stem cells of small intestinal crypts: where are they?Cell proliferation · 2009Article
- Doublecortin and CaM kinase-like-1 and leucine-rich-repeat-containing G-protein-coupled receptor mark quiescent and cycling intestinal stem cells, respectively.Stem cells (Dayton, Ohio) · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Colonic epithelial stem cells are believed to be located at the crypt base where they have previously been shown to express musashi-1. The colonic stem cell niche, which includes extracellular matrix and myofibroblasts (together with other cell types), is likely to be important in maintaining the function of the progenitor cells. The aims of our studies were to characterize stem cells in isolated and disaggregated human colonic crypt epithelial cells and investigate their interactions with monolayers of primary human colonic myofibroblasts. In unfractionated preparations of disaggregated colonic crypts, musashi-1 positive cells preferentially adhered to colonic myofibroblasts, despite the presence of excess blocking anti-beta(1)-integrin antibody. These adherent epithelial cells remained viable for a number of days and developed slender processes. Cells with side population characteristics (as demonstrated by ability to expel the dye Hoechst 33342) were consistently seen in the isolated colonic crypt epithelial cells. These side population cells expressed musashi-1, beta(1)-integrin, BerEP4, and CD133. Sorted side population crypt epithelial cells also rapidly adhered to primary colonic myofibroblasts. In conclusion, in preparation of isolated and disaggregated human colonic crypts, cells with stem cell characteristics preferentially adhere to primary human colonic myofibroblasts in a beta(1)-integrin-independent fashion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.