ArticleMolecular biology of the cell2009
Sec6p anchors the assembled exocyst complex at sites of secretion.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Emerging Roles of Exocyst Complex in Fungi: A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- Article
- A WICB 50th Favorite: Sec6p anchors the assembled exocyst complex at sites of secretion.Molecular biology of the cell · 2022Article
- Integrative structure and function of the yeast exocyst complex.Protein science : a publication of the Protein Society · 2020Article
- The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge.Journal of cell science · 2019Article
- Exposing the Elusive Exocyst Structure.Trends in biochemical sciences · 2018Review
- The Par3 polarity protein is an exocyst receptor essential for mammary cell survival.Nature communications · 2017Article
- Crystal structure of Sec10, a subunit of the exocyst complex.Scientific reports · 2017Article
- Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex.Nature structural & molecular biology · 2016Article
- Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies.Frontiers in cell and developmental biology · 2016Article
- Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by Magnaporthe oryzae.The Plant cell · 2015Article
- The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching.Eukaryotic cell · 2015Article
- Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in both plants and animals.Molecular biology of the cell · 2014Article
- The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells.Frontiers in plant science · 2014Review
- CAPS and Munc13: CATCHRs that SNARE Vesicles.Frontiers in endocrinology · 2013Review
- Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana.Molecular biology of the cell · 2013Article
- Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.Traffic (Copenhagen, Denmark) · 2012Article
- Exorcising the exocyst complex.Traffic (Copenhagen, Denmark) · 2012Review
- Evolution of the land plant exocyst complexes.Frontiers in plant science · 2012Article
- Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The exocyst is an essential protein complex required for targeting and fusion of secretory vesicles to sites of exocytosis at the plasma membrane. To study the function of the exocyst complex, we performed a structure-based mutational analysis of the Saccharomyces cerevisiae exocyst subunit Sec6p. Two "patches" of highly conserved residues are present on the surface of Sec6p; mutation of either patch does not compromise protein stability. Nevertheless, replacement of SEC6 with the patch mutants results in severe temperature-sensitive growth and secretion defects. At nonpermissive conditions, although trafficking of secretory vesicles to the plasma membrane is unimpaired, none of the exocyst subunits are polarized. This is consistent with data from other exocyst temperature-sensitive mutants, which disrupt the integrity of the complex. Surprisingly, however, these patch mutations result in mislocalized exocyst complexes that remain intact. Our results indicate that assembly and polarization of the exocyst are functionally separable events, and that Sec6p is required to anchor exocyst complexes at sites of secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.