ArticlePlant & cell physiology2009
AtAMT1;4, a pollen-specific high-affinity ammonium transporter of the plasma membrane in Arabidopsis.
Article in Plant & cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 110 citations in OpenAlex.
- Comprehensive analysis of cotton AMT genes and functional analysis of the GhAMT1.4a in nitrogen metabolism.Functional & integrative genomics · 2026Article
- Genome-Wide Analysis of Ammonium Transporter Genes in Flowering Chinese Cabbage and Functional Insights into BcAMT1.1 Under Low-Nitrogen Conditions.Plants (Basel, Switzerland) · 2025Article
- Genome-wide analysis of the ammonium transporters gene family in Phaseolus vulgaris and its roles in response to drought and salinity stress.Scientific reports · 2025Article
- Ammonium Transporter 1 (Current issues in molecular biology · 2025Article
- EMBRYONIC FLOWER 1 regulates male reproduction by repressing the jasmonate pathway downstream transcription factor MYB26.The Plant cell · 2024Article
- The Identification ofPlants (Basel, Switzerland) · 2024Article
- Article
- The Root-Colonizing EndophyteInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed (Genes · 2023Article
- Genome-Wide Identification and Expression Analysis of the Ammonium Transporter Family Genes in Soybean.International journal of molecular sciences · 2023Article
- Effects of concentration-dependent graphene on maize seedling development and soil nutrients.Scientific reports · 2023Article
- Identification and functional characterization of the sugarcane (Frontiers in plant science · 2023Article
- Genome-Wide Identification of AMT2-Type Ammonium Transporters Reveal That CsAMT2.2 and CsAMT2.3 Potentially Regulate NHInternational journal of molecular sciences · 2022Article
- Genome-Wide Identification and Expression Analysis ofInternational journal of molecular sciences · 2022Article
- Functional characterization of the sugarcane (Frontiers in plant science · 2022Article
- Contrasting anther glucose-6-phosphate dehydrogenase activities between two bean varieties suggest an important role in reproductive heat tolerance.Plant, cell & environment · 2021Article
- Article
- A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze].BMC plant biology · 2020Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- Functional Characterization of theFrontiers in plant science · 2020Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen represents an important nitrogen sink in flowers to ensure pollen viability. Since pollen cells are symplasmically isolated during maturation and germination, membrane transporters are required for nitrogen import across the pollen plasma membrane. This study describes the characterization of the ammonium transporter AtAMT1;4, a so far uncharacterized member of the Arabidopsis AMT1 family, which is suggested to be involved in transporting ammonium into pollen. The AtAMT1;4 gene encodes a functional ammonium transporter when heterologously expressed in yeast or when overexpressed in Arabidopsis roots. Concentration-dependent analysis of (15)N-labeled ammonium influx into roots of AtAMT1;4-transformed plants allowed characterization of AtAMT1;4 as a high-affinity transporter with a K(m) of 17 microM. RNA and protein gel blot analysis showed expression of AtAMT1;4 in flowers, and promoter-gene fusions to the green fluorescent protein (GFP) further defined its exclusive expression in pollen grains and pollen tubes. The AtAMT1;4 protein appeared to be localized to the plasma membrane as indicated by protein gel blot analysis of plasma membrane-enriched membrane fractions and by visualization of GFP-tagged AtAMT1;4 protein in pollen grains and pollen tubes. However, no phenotype related to pollen function could be observed in a transposon-tagged line, in which AtAMT1;4 expression is disrupted. These results suggest that AtAMT1;4 mediates ammonium uptake across the plasma membrane of pollen to contribute to nitrogen nutrition of pollen via ammonium uptake or retrieval.
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