Evidence map›Paper›PMID 19073133›Full record

ArticleAnalytical biochemistry2009

Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassays.

Henryk Szmacinski, Krishanu Ray, Joseph R Lakowicz

Abstract read
In one paragraph

Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Fluoxetine competes with cortisol for binding to human serum albumin.Iranian journal of pharmaceutical research : IJPR · 2012
    Article
  12. Feasibility of Using Bimetallic Plasmonic Nanostructures to Enhance the Intrinsic Emission of Biomolecules.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011
    Article
  13. Metallic-Nanostructure-Enhanced Fluorescence of Single Flavin Cofactor and Single Flavoenzyme Molecules.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Henryk SzmacinskiCenter for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA. henry@cfs.umbi.umd.edu
Krishanu Ray
Joseph R Lakowicz

Funding

Metallic Surfaces and Particles in DNA AnalysisR01HG002655 · NHGRI · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2003 to 2012
$2.8M
Plasmon-Controlled Fluorescence and Cardiac MarkersR01EB006521 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2007 to 2015
$2.7M
NHGRI NIH HHS HG002655NHGRI NIH HHS R01 HG002655NIBIB NIH HHS EB006521NIBIB NIH HHS R01 EB006521
6 · The paper itself

Abstract

The detection of submonolayers of proteins based on native fluorescence is a potentially valuable approach for label-free detection. We have examined the possibility of using silver nanostructures to increase the emission of tryptophan residues in proteins. Fluorescence spectra, intensities, and lifetimes of multilayers and submonolayers of proteins deposited on the surfaces of silver island films were measured. Increased fluorescence intensities from two- to three-fold and similar decreases in lifetimes were observed in the presence of the silver nanoparticles compared with the proteins on the surface of the bare quartz. The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores.

Indexed as

Metal NanoparticlesMolecular Probe TechniquesSilverFluorescenceProteinsTryptophanProteinsSilverTryptophan

Identifiers

PMID19073133
PMCPMC2737412

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.