ArticleAnalytical chemistry2009
Quantitative proteomics: measuring protein synthesis using 15N amino acid labeling in pancreatic cancer cells.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Hyperthermia Influences the Secretion Signature of Tumor Cells and Affects Endothelial Cell Sprouting.Biomedicines · 2023Article
- Cell-type-specific quantification of protein synthesis in vivo.Nature protocols · 2019Article
- Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation.JOP : Journal of the pancreas · 2013Article
- Platform dependencies in bottom-up hydrogen/deuterium exchange mass spectrometry.Molecular & cellular proteomics : MCP · 2013Article
- Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells.Experimental hematology & oncology · 2013Article
- Plasma proteome dynamics: analysis of lipoproteins and acute phase response proteins with 2H2O metabolic labeling.Molecular & cellular proteomics : MCP · 2012Article
- Inhibition of glycogen phosphorylation induces changes in cellular proteome and signaling pathways in MIA pancreatic cancer cells.Pancreas · 2012Article
- Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis.Journal of proteome research · 2012Article
- SILAM for quantitative proteomics of liver Akt1/PKBα after burn injury.International journal of molecular medicine · 2012Article
- Measuring protein synthesis using metabolic ²H labeling, high-resolution mass spectrometry, and an algorithm.Analytical biochemistry · 2011Article
- Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research.Proteomics. Clinical applications · 2011Review
- Proteomics in pancreatic cancer research.International journal of proteomics · 2011Article
- Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis.Electrophoresis · 2010Article
- Inhibition of protein phosphorylation in MIA pancreatic cancer cells: confluence of metabolic and signaling pathways.Journal of proteome research · 2010Article
- Profiling pancreatic cancer-secreted proteome using 15N amino acids and serum-free media.Pancreas · 2010Article
- Quantitative proteomics and biomarker discovery in human cancer.Expert review of proteomics · 2009Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Pancreatic cancer MIA PaCa cells were cultured in the presence and absence of (15)N amino acids mixture for 72 h. During protein synthesis, the incorporation of (15)N amino acids results in a new mass isotopomer distribution in protein, which is approximated by the concatenation of two binomial distributions of (13)C and (15)N. The fraction of protein synthesis (FSR) can thus be determined from the relative intensities of the "labeled" (new) and the "unlabeled" (old) spectra. Six prominent spots were picked from 2-D gels of proteins from lysates of cells cultured in 0% (control), 50%, and 33% (15)N enriched media. These protein spots were digested and analyzed with matrix-assisted laser desorption ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry. The isotopomer distribution of peptides after labeling can be fully accounted for by the labeled (new) and unlabeled (old) peptides. The ratio of the new and old peptide fractions was determined using multiple regression analysis of the observed spectrum as a linear combination of the expected new and the old spectra. The fractional protein synthesis rates calculated from such ratios of the same peptide from cells grown in 50% and 33% (15)N amino acid enrichments were comparable to each other. The FSR of these six identified proteins ranged between 44 and 76%.
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