ArticlePLoS biology2008
Epigenomic consequences of immortalized plant cell suspension culture.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 209 citations in OpenAlex.
- A plant cell-based platform for the expression of complex proteins with fucose-reduced sialylated N-glycans.Plant biotechnology journal · 2026Article
- The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins-A Case Study About Producing a Rice Enzyme.International journal of molecular sciences · 2025Article
- Dynamic changes in DNA methylation play a regulatory role in gene expression during the formation of callus from immature barley embryos.BMC plant biology · 2025Article
- An Arabidopsis Cell Culture With Weak Circadian Rhythms Under Constant Light Compared With Constant Dark Can Be Rescued by ELF3.Plant direct · 2024Article
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- The roles of epigenetic regulators in plant regeneration: Exploring patterns amidst complex conditions.Plant physiology · 2024Review
- Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time.Scientific reports · 2024Article
- Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Epigenomics in stress tolerance of plants under the climate change.Molecular biology reports · 2023Review
- Somaclonal Variation-Advantage or Disadvantage in Micropropagation of the Medicinal Plants.International journal of molecular sciences · 2023Review
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- Optimization of Genome Knock-In Method: Search for the Most Efficient Genome Regions for Transgene Expression in Plants.International journal of molecular sciences · 2022Review
- Harnessing epigenetic variability for crop improvement: current status and future prospects.Genes & genomics · 2022Review
- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Will epigenetics be a key player in crop breeding?Frontiers in plant science · 2022Review
- Dynamic changes in DNA methylation occur in TE regions and affect cell proliferation during leaf-to-callus transition in Arabidopsis.Epigenetics · 2022Article
- Elevated transcription of transposable elements is accompanied by het-siRNA-driven de novo DNA methylation in grapevine embryogenic callus.BMC genomics · 2021Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Androgenic-Induced Transposable Elements Dependent Sequence Variation in Barley.International journal of molecular sciences · 2021Article
- The epiallelic potential of transposable elements and its evolutionary significance in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundance of histone and DNA modifications in a continuously proliferating, dedifferentiated cell suspension culture of Arabidopsis. We have found that euchromatin becomes hypermethylated in culture and that a small percentage of the hypermethylated genes become associated with heterochromatic marks. In contrast, the heterochromatin undergoes dramatic and very precise DNA hypomethylation with transcriptional activation of specific transposable elements (TEs) in culture. High throughput sequencing of small interfering RNA (siRNA) revealed that TEs activated in culture have increased levels of 21-nucleotide (nt) siRNA, sometimes at the expense of the 24-nt siRNA class. In contrast, TEs that remain silent, which match the predominant 24-nt siRNA class, do not change significantly in their siRNA profiles. These results implicate RNA interference and chromatin modification in epigenetic restructuring of the genome following the activation of TEs in immortalized cell culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.