ArticleVirology2009
Growth of an RNA virus in single cells reveals a broad fitness distribution.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 95 citations in OpenAlex.
- Coevolutionary dynamics of viruses and their defective interfering particles.PLoS computational biology · 2026Article
- Article
- From spark to wildfire: illuminating single-cell origins of virus infection spread and diversification.bioRxiv : the preprint server for biology · 2025Article
- HSV-1 progeny production by individual human keratinocytes spans three orders of magnitude.Journal of virology · 2025Article
- Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Direct relationship between protein expression and progeny yield of herpes simplex virus 1.mBio · 2025Article
- Navigating the Purification Process: Maintaining the Integrity of Replication-Competent Enveloped Viruses.Vaccines · 2025Review
- Abortive Infection of Animal Cells: What Goes Wrong.Annual review of virology · 2024Review
- All the single cells: if you like it then you should put some virus on it.Journal of virology · 2024Review
- Single-cell herpes simplex virus type 1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics.Science advances · 2024Article
- Stochastic model of vesicular stomatitis virus replication reveals mutational effects on virion production.PLoS computational biology · 2024Article
- SARS-CoV-2 infection triggers pro-atherogenic inflammatory responses in human coronary vessels.Nature cardiovascular research · 2023Article
- Influenza virus transcription and progeny production are poorly correlated in single cells.eLife · 2023Article
- Cellular state landscape and herpes simplex virus type 1 infection progression are connected.Nature communications · 2023Article
- Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Single-Cell Infection of Influenza A Virus Using Drop-Based Microfluidics.Microbiology spectrum · 2022Article
- Virus-like Particles: Measures and Biological Functions.Viruses · 2022Review
- Heterogeneity in the Response of Different Subtypes ofViruses · 2021Article
- Single Cell Analysis of Drug Susceptibility ofAntibiotics (Basel, Switzerland) · 2020Article
- Kinetics of Oncolytic Reovirus T3D Replication and Growth Pattern in Mesenchymal Stem Cells.Cell journal · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Genetic and environmental factors will influence the growth of an RNA virus, but their relative contributions are challenging to resolve because standard culture methods mask how virus particles interact with individual host cells. Here, single particles of vesicular stomatitis virus, a prototype RNA virus, were used to infect individual BHK cells. Infected cells produced 50 to 8000 progeny virus particles, but these differences were lost upon subsequent culture, suggesting the diversity of yields reflected cell-to-cell differences rather than viral genetic variation. Cells infected at different phases of their cell cycle produced from 1400 (early S) to 8700 (G(2)M) infectious virus particles, coinciding with the middle-to-upper range of the observed distribution. Fluctuations in virus and cell compositions and noisy gene expression may also contribute to the broad distribution of virus yields. These findings take a step toward quantifying how environmental variation can impact the fitness distribution of an RNA virus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.