ArticleJournal of the American Society for Mass Spectrometry2009
Tandem mass spectrometry investigation of ADP-ribosylated kemptide.
Article in Journal of the American Society for Mass Spectrometry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
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Who cites it
24 citing papers in PubMed.
- In-Source Collision-Induced Dissociation (CID) Improves Higher-Energy Collisional Dissociation (HCD)-Dependent Fragmentation of ADP-Ribosyl Peptides.Rapid communications in mass spectrometry : RCM · 2025Article
- A Combined Gas-Phase Separation Strategy for ADP-ribosylated Peptides.Journal of the American Society for Mass Spectrometry · 2023Article
- PARPs and ADP-Ribosylation in Chronic Inflammation: A Focus on Macrophages.Pathogens (Basel, Switzerland) · 2023Review
- MSFragger-Labile: A Flexible Method to Improve Labile PTM Analysis in Proteomics.Molecular & cellular proteomics : MCP · 2023Article
- Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genome.Communications biology · 2022Article
- A Novel Spectral Annotation Strategy Streamlines Reporting of Mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-γ.Molecular & cellular proteomics : MCP · 2022Article
- The RiboMaP Spectral Annotation Method Applied to Various ADP-Ribosylome Studies Including INF-γ-Stimulated Human Cells and Mouse Tissues.Frontiers in cardiovascular medicine · 2022Article
- A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation.Journal of proteome research · 2019Article
- Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry.Methods in enzymology · 2019Article
- Mass spectrometry for serine ADP-ribosylation? Think o-glycosylation!Nucleic acids research · 2017Article
- PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation.Nature communications · 2016Article
- Nudix hydrolases degrade protein-conjugated ADP-ribose.Scientific reports · 2015Article
- The Promise of Proteomics for the Study of ADP-Ribosylation.Molecular cell · 2015Article
- Proteomics approaches to identify mono-(ADP-ribosyl)ated and poly(ADP-ribosyl)ated proteins.Proteomics · 2015Article
- The Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation.Nature communications · 2014Article
- Aryl hydrocarbon receptor activation by dioxin targets phosphoenolpyruvate carboxykinase (PEPCK) for ADP-ribosylation via 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly(ADP-ribose) polymerase (TiPARP).The Journal of biological chemistry · 2013Article
- Identification of ADP-ribosylation sites of CD38 mutants by precursor ion scanning mass spectrometry.Analytical biochemistry · 2013Article
- Molecular and biological characterization of Streptococcal SpyA-mediated ADP-ribosylation of intermediate filament protein vimentin.The Journal of biological chemistry · 2012Article
- A Review of Tandem Mass Spectrometry Characterization of Adenosine Diphosphate-Ribosylated Peptides.International journal of mass spectrometry · 2012Article
- ADP-ribosylation of arginine.Amino acids · 2011Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Bacterial adenosine diphosphate-ribosyltransferases (ADPRTs) are toxins that play a significant role in pathogenicity by inactivating host proteins through covalent addition of ADP-ribose. In this study we used ADP-ribosylated Kemptide (LRRASLG) as a standard to examine the effectiveness of three common tandem mass spectrometry fragmentation methods for assignment of amino acid sequence and site of modification. Fragmentation mechanisms investigated include low-energy collision-induced dissociation (CID), infrared multiphoton dissociation (IRMPD), and electron-capture dissociation (ECD); all were performed on a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer. We show that ECD, but neither CID nor IRMPD, of ADP-ribosylated Kemptide produces tandem mass spectra that are interpretable with regard to amino acid sequence assignment and site of modification. Examination of CID and IRMPD tandem mass spectra of ADP-ribosylated Kemptide revealed that fragmentation was primarily focused to the ADP-ribose region, generating several potential diagnostic ions for use in discovery of ADP-ribosylated proteins. Because of the lower relative sensitivity of ECD during data-dependent acquisition to CID, we suggest a 2-fold strategy where CID and IRMPD are first used to detect ADP-ribosylated peptides, followed by sequence assignment and location of modification by ECD analysis.
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Registered trials
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