ArticleCell transplantation2008
Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells.
Article in Cell transplantation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
- Article
- Injectable BMSC-Based Extracellular Matrix-Mimicking Microtissue for Myocardial Infarction Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Targeted delivery of engineered adipose-derived stem cell secretome to promote cardiac repair after myocardial infarction.Journal of controlled release : official journal of the Controlled Release Society · 2025Article
- Rebuilding the myocardial microenvironment to enhance mesenchymal stem cells-mediated regeneration in ischemic heart disease.Frontiers in bioengineering and biotechnology · 2024Review
- Mesenchymal Stromal Cells Overexpressing Farnesoid X Receptor Exert Cardioprotective Effects Against Acute Ischemic Heart Injury by Binding Endogenous Bile Acids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Mesenchymal stem cells for critical limb ischemia: their function, mechanism, and therapeutic potential.Stem cell research & therapy · 2022Review
- MicroRNA124 and microRNA21-5p regulate migration, proliferation and differentiation of rat bone marrow mesenchymal stem cells.Bioscience reports · 2020Article
- Review
- Improving the engraftment and integration of cell transplantation for cardiac regeneration.Cardiovascular research · 2020Article
- Immune Dysregulation in HFpEF: A Target for Mesenchymal Stem/Stromal Cell Therapy.Journal of clinical medicine · 2020Review
- Extracellular Vesicles as Therapeutic Agents for Cardiac Fibrosis.Frontiers in physiology · 2020Review
- Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis.Stem cells international · 2020Review
- Inflammation in myocardial injury: mesenchymal stem cells as potential immunomodulators.American journal of physiology. Heart and circulatory physiology · 2019Review
- The Immunomodulatory Functions of Mesenchymal Stromal/Stem Cells Mediated via Paracrine Activity.Journal of clinical medicine · 2019Review
- Concise Review: Rational Use of Mesenchymal Stem Cells in the Treatment of Ischemic Heart Disease.Stem cells translational medicine · 2018Review
- CCN5/WISP2 and metabolic diseases.Journal of cell communication and signaling · 2018Review
- Clinical significance of ALDH1 combined with DAPI expression in patients with esophageal carcinoma.Oncology letters · 2017Article
- Regulation of focal adhesion turnover in SDF-1α-stimulated migration of mesenchymal stem cells in neural differentiation.Scientific reports · 2017Article
- Optimizing stem cells for cardiac repair: Current status and new frontiers in regenerative cardiology.World journal of stem cells · 2017Review
- New Trends in Heart Regeneration: A Review.Journal of stem cells & regenerative medicine · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Therapeutic implantation of mesenchymal stem cells (MSCs) is entering the realm of clinical trials for several human diseases, and yet much remains uncertain regarding their dynamic distribution and cell fate after in vivo application. Discrepancies in the literature can be attributed in part to the use of different cell labeling/tracking methods and cell administration protocols. To identify a stem cell detection method suitable for myocardial implantation in a large animal model, we experimented on three different MSC labeling methods: adenovirus-mediated expression of enhanced green fluorescence protein (EGFP) and beta-galactosidase (LacZ), and nuclear staining with DAPI. Intramuscular and intracoronary administrations of labeled porcine MSCs identified the nuclear affinity dye to be a reliable stem cell tracking marker. Stem cell identification is facilitated by an optimized live cell labeling condition generating bright blue fluorescence sharply confined to the nucleus. DAPI-labeled MSCs retained full viability, ceased proliferation, and exhibited an increased differentiation potential. The labeled MSCs remained fully active in expressing key growth factor and cytokine genes, and notably exhibited enhanced expression of the chemokine receptor CXCR4 and its ligand SDF1, indicating their competency in response to tissue injury. Histological analysis revealed that approximately half a million MSCs or approximately 2% of the administered MSCs remained localized in the normal pig heart 2 weeks after coronary infusion. That the vast majority of these identified MSCs were interstitial indicated the ability of MSCs to migrate across the coronary endothelium. No evidence was obtained indicating MSC differentiation to cardiomyocyte.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.