ArticleDevelopmental neurobiology
Proneural gene ash1 promotes amacrine cell production in the chick retina.
Article in Developmental neurobiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Pluripotent Stem Cell-Based Approaches to Explore and Treat Optic Neuropathies.Frontiers in neuroscience · 2018Review
- Ascl1 as a novel player in the Ptf1a transcriptional network for GABAergic cell specification in the retina.PloS one · 2014Article
- Heterochronic misexpression of Ascl1 in the Atoh7 retinal cell lineage blocks cell cycle exit.Molecular and cellular neurosciences · 2013Article
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Production of high-titer RCAS retrovirus.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Ascl1 expression defines a subpopulation of lineage-restricted progenitors in the mammalian retina.Development (Cambridge, England) · 2011Article
- Generating retinal neurons by reprogramming retinal pigment epithelial cells.Expert opinion on biological therapy · 2010Review
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Using neurogenin to reprogram chick RPE to produce photoreceptor-like neurons.Investigative ophthalmology & visual science · 2010Article
- Pro-photoreceptor activity of chick neurogenin1.Investigative ophthalmology & visual science · 2009Article
- Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.Stem cells (Dayton, Ohio) · 2009Article
- Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.Molecular vision · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The diverse types of neurons and Müller glia in the vertebrate retina are believed to arise from common progenitor cells. To better understand how neural diversity is achieved during retinal neurogenesis, we examined the function of ash1, a proneural bHLH gene expressed in progenitor cells throughout retinal neurogenesis. Published studies using retinal explant culture derived from knockout mice concluded that ash1 is required for the production of late-born neurons, including bipolar cells. In this study, gain-of-function experiments were carried out in ovo in embryonic chick retina. In the developing chick retina, expression of ash1 temporally overlapped with, but spatially differed from, the expression of ngn2, also a proneural gene expressed in progenitor cells throughout retinal neurogenesis. Retrovirus-driven overexpression of ash1 in the developing chick retina decreased the progenitor population (BrdU+ or expressing ngn2), expanded the amacrine population (AP2alpha+ or Pax6+), and reduced bipolar (chx10 mRNA+) and Müller glial (vimentin+) populations. Photoreceptor deficiency occurred after the completion of neurogenesis. The number of ganglion cells, which are born first during retinal neurogenesis, remained unchanged. Similar overexpression of ngn2 did not produce discernible changes in retinal neurogenesis, nor in ash1 expression. These results suggest that ash1 promotes the production of amacrine cells and thus may participate in a regulatory network governing neural diversity in the chick retina.
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