Evidence map›Paper›PMID 19067179›Full record

ArticleJournal of biomolecular NMR2009

Measurement of imino 1H-1H residual dipolar couplings in RNA.

Michael P Latham, Arthur Pardi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Michael P LathamDepartment of Chemistry and Biochemistry, 215 UCB, University of Colorado, Boulder, CO 80309-0215, USA.
Arthur Pardi
University of Colorado Boulder · USUniversity of Colorado System · US

Funding

Predoctoral Training Molecular BiophysicsT32GM065103 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 2002 to 2021
$4.3M
Structure and Dynamics of RNAR01AI033098 · NIAID · UNIVERSITY OF COLORADO AT BOULDER · PI PARDI, ARTHUR · 1992 to 2006
$1.7M
Structure and Dynamics of RNA and Protein-RNA ComplexesR56AI033098 · NIAID · UNIVERSITY OF COLORADO · PI PARDI, ARTHUR · 2008 to 2008
$295k
PURCHASE OF 600 MHZ NMR AND UPGRADE OF NMR FACILITIESS10RR011969 · NCRR · UNIVERSITY OF COLORADO AT BOULDER · PI PARDI, ARTHUR · 1997 to 1997
–
NCRR NIH HHS S10 RR011969NIAID NIH HHS AI33098NIAID NIH HHS R01 AI033098NIAID NIH HHS R56 AI033098NIGMS NIH HHS T32 GM065103NIGMS NIH HHS T32 GM65103
6 · The paper itself

Abstract

Imino (1)H-(15)N residual dipolar couplings (RDCs) provide additional structural information that complements standard (1)H-(1)H NOEs leading to improvements in both the local and global structure of RNAs. Here, we report measurement of imino (1)H-(1)H RDCs for the Iron Responsive Element (IRE) RNA and native E. coli tRNA(Val) using a BEST-Jcomp-HMQC2 experiment. (1)H-(1)H RDCs are observed between the imino protons in G-U wobble base pairs and between imino protons on neighboring base pairs in both RNAs. These imino (1)H-(1)H RDCs complement standard (1)H-(15)N RDCs because the (1)H-(1)H vectors generally point along the helical axis, roughly perpendicular to (1)H-(15)N RDCs. The use of longitudinal relaxation enhancement increased the signal-to-noise of the spectra by ~3.5-fold over the standard experiment. The ability to measure imino (1)H-(1)H RDCs offers a new restraint, which can be used in NMR domain orientation and structural studies of RNAs.

Indexed as

Escherichia coliIminesIron-Regulatory ProteinsNitrogen IsotopesNuclear Magnetic Resonance, BiomolecularRNARNA, BacterialRNA, Transfer, ValIminesIron-Regulatory ProteinsNitrogen IsotopesRNARNA, BacterialRNA, Transfer, Val

Identifiers

PMID19067179
PMCPMC2846714
OpenAlexW2052675539

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.