ArticleChromosoma2009
CAF-1 is required for efficient replication of euchromatic DNA in Drosophila larval endocycling cells.
Article in Chromosoma, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Structural Basis for the Interaction Between Yeast Chromatin Assembly Factor 1 and Proliferating Cell Nuclear Antigen.Journal of molecular biology · 2024Article
- Cellular polyploidy in organ homeostasis and regeneration.Protein & cell · 2023Article
- CAF-1 deposits newly synthesized histones during DNA replication using distinct mechanisms on the leading and lagging strands.Nucleic acids research · 2023Article
- Histone deposition promotes recombination-dependent replication at arrested forks.PLoS genetics · 2019Article
- ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.Epigenetics & chromatin · 2018Article
- Replication-Coupled Nucleosome Assembly in the Passage of Epigenetic Information and Cell Identity.Trends in biochemical sciences · 2018Review
- Article
- CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non-small cell lung cancer cells by inducing G0/G1 cell cycle arrest.Experimental and therapeutic medicine · 2017Article
- Emerging roles of the histone chaperone CAF-1 in cellular plasticity.Current opinion in genetics & development · 2017Review
- Article
- Maintenance of Epigenetic Information.Cold Spring Harbor perspectives in biology · 2016Review
- Article
- Replicating Nucleosomes.Science advances · 2015Article
- A unique binding mode enables MCM2 to chaperone histones H3-H4 at replication forks.Nature structural & molecular biology · 2015Article
- Histone storage and deposition in the early Drosophila embryo.Chromosoma · 2015Review
- A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.PloS one · 2015Article
- Histone chaperone CAF-1: essential roles in multi-cellular organism development.Cellular and molecular life sciences : CMLS · 2015Review
- A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli.Molecular biology of the cell · 2014Article
- Chromatin and DNA replication.Cold Spring Harbor perspectives in biology · 2013Review
- Endoreplication and polyploidy: insights into development and disease.Development (Cambridge, England) · 2013Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endocycle constitutes an effective strategy for cell growth during development. In contrast to the mitotic cycle, it consists of multiple S-phases with no intervening mitosis and lacks a checkpoint ensuring the replication of the entire genome. Here, we report an essential requirement of chromatin assembly factor-1 (CAF-1) for Drosophila larval endocycles. This complex promotes histone H3-H4 deposition onto newly synthesised DNA in vitro. In metazoans, the depletion of its large subunit leads to the rapid accumulation of cells in S-phase. However, whether this slower S-phase progression results from the activation of cell cycle checkpoints or whether it reflects a more direct requirement of CAF-1 for efficient replication in vivo is still debated. Here, we show that, strikingly, Drosophila larval endocycling cells depleted for the CAF-1 large subunit exhibit normal dynamics of progression through endocycles, although accumulating defects, such as perturbation of nucleosomal organisation, reduction of the replication efficiency of euchromatic DNA and accumulation of DNA damage. Given that the endocycle lacks a checkpoint ensuring the replication of the entire genome, the biological context of Drosophila larval development offered a unique opportunity to highlight the requirement of CAF-1 for chromatin organisation and efficient replication processes in vivo, independently of checkpoint activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.