ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma.Journal of nanobiotechnology · 2022Article
- Tissue-engineered sling with adipose-derived stem cells under static mechanical strain.Experimental and therapeutic medicine · 2017Article
- Epidermal Growth Factor (EGF)-Like Repeats and Discoidin I-Like Domains 3 (EDIL3): A Potential New Therapeutic Tool for the Treatment of Keloid Scars.Tissue engineering and regenerative medicine · 2017Article
- Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical andBiological procedures online · 2017Article
- Adipose-derived stem cells seeded on polyglycolic acid for the treatment of stress urinary incontinence.World journal of urology · 2016Article
- A comparative study of non-viral gene delivery techniques to human adipose-derived mesenchymal stem cell.International journal of molecular sciences · 2014Article
- Isolation of human adipose-derived stromal cells using laser-assisted liposuction and their therapeutic potential in regenerative medicine.Stem cells translational medicine · 2013Article
- PPARγ silencing enhances osteogenic differentiation of human adipose-derived mesenchymal stem cells.Journal of cellular and molecular medicine · 2013Article
- Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate.International journal of oral science · 2013Article
- CD90 (Thy-1)-positive selection enhances osteogenic capacity of human adipose-derived stromal cells.Tissue engineering. Part A · 2013Article
- Direct head-to-head comparison of cationic liposome-mediated gene delivery to mesenchymal stem/stromal cells of different human sources: a comprehensive study.Human gene therapy methods · 2013Article
- Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3.BMC biotechnology · 2012Article
- Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells.Biological procedures online · 2012Article
- Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures.Journal of cellular and molecular medicine · 2012Article
- Low-power GaAlAs laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via IGF1 and BMP2.PloS one · 2012Article
- Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells.BMC biotechnology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells are a promising resource for gene therapy. Adipose tissue-derived stem cells (ADSCs) offer advantages because of their abundance and ease of isolation. However, it is difficult to transduce genes into ADSCs by common transfection methods, especially nonviral methods. We report here the use of a new electroporation method, termed "microporation," to transduce plasmids into human ADSCs (hADSCs). We determined optimal conditions that led to efficient transfection of >76.1% of the microporated hADSCs with only minimal cell damage or cytotoxicity. We demonstrated the expression of both enhanced green fluorescent protein (EGFP) and luciferase from different promoters in microporated hADSCs. More important, the microporated hADSCs retained their multipotency and reporter gene expression was maintained for >2 weeks in vitro and in vivo. We further showed that a Tet-ON-inducible gene expression system could be microporated into hADSCs and that this system was functional following transplantation of the microporated cells into nude mice. Taken together, our data demonstrate that microporation allows a highly efficient transfection of hADSCs, without impairing their stem cell properties.
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