ArticleJournal of visualized experiments : JoVE2008
In vitro differentiation of mouse embryonic stem (mES) cells using the hanging drop method.
Article in Journal of visualized experiments : JoVE, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Cardiovascular Organoids With Adjustable Endothelial Composition via SOX17-Engineered hPSCs.Biotechnology and bioengineering · 2026Article
- Unveiling FLNC variants: iPSC-derived myogenic cells as a model to study disease mechanisms.Skeletal muscle · 2026Article
- Modeling combinatorial regulation from single-cell multi-omics provides regulatory units underpinning cell type landscape using cRegulon.Genome biology · 2025Article
- The sotos syndrome gene Nsd1 safeguards developmental gene enhancers poised for transcription by maintaining the precise deposition of histone methylation.The Journal of biological chemistry · 2025Article
- Modeling the pre-metastatic niche of gastric cancer peritoneal metastasis under spatiotemporal resolution and investigating EVs-mediated immune suppression.Frontiers in immunology · 2025Review
- Redox heterogeneity in mouse embryonic stem cells individualizes cell fate decisions.Developmental cell · 2024Article
- Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells.eLife · 2024Article
- Data integration and inference of gene regulation using single-cell temporal multimodal data with scTIE.Genome research · 2024Article
- Biofabrication of prevascularized spheroids for bone tissue engineering by fusion of microvascular fragments with osteoblasts.Frontiers in bioengineering and biotechnology · 2024Article
- Thermal inkjet bioprinting drastically alters cell phenotype.Biofabrication · 2023Article
- scTIE: data integration and inference of gene regulation using single-cell temporal multimodal data.bioRxiv : the preprint server for biology · 2023Article
- Liver-on-a-chip: Considerations, advances, and beyond.Biomicrofluidics · 2022Review
- Three-dimensional in vitro culture models in oncology research.Cell & bioscience · 2022Review
- Personalized Medicine Approach in a DCM Patient withJournal of personalized medicine · 2022Article
- DNA methylation-independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins.Life science alliance · 2022Article
- Rat-Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Long-term arsenic exposure impairs differentiation in mouse embryonal stem cells.Journal of applied toxicology : JAT · 2021Article
- Arginine methylation of METTL14 promotes RNA NNature communications · 2021Article
- Implications of the Wilms' Tumor Suppressor Wt1 in Cardiomyocyte Differentiation.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells have the remarkable potential to develop into many different cell types. When a stem cell divides, each new cell has the potential to either remain a stem cell or become another type of cell with a more specialized function, This promising of science is leading scientists to investigate the possibility of cell-based therapies to treat disease. When culture in suspension without antidifferentiation factors, embryonic stem cells spontaneously differentiate and form three-dimensional multicellular aggregates. These cell aggregates are called embryoid bodies(EB). Hanging drop culture is a widely used EB formation induction method. The rounded bottom of hanging drop allows the aggregation of ES cells which can provide mES cells a good environment for forming EBs. The number of ES cells aggregatied in a hanging drop can be controlled by varying the number of cells in the initial cell suspension to be hung as a drop from the lid of Petri dish. Using this method we can reproducibly form homogeneous EBs from a predetermined number of ES cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.