ArticleJournal of cell science2009
Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Distinct Rab11-associated membrane trafficking pathways bidirectionally control neuronal extracellular vesicle cargo traffic.bioRxiv : the preprint server for biology · 2026Article
- A Potential Role of EFR3A in Human Disease States.Biomolecules · 2025Review
- Efr3b is essential for social recognition by modulating the excitability of CA2 pyramidal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The CHD family chromatin remodeling enzyme, Kismet, promotes both clathrin-mediated and activity-dependent bulk endocytosis.PloS one · 2024Article
- EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes.Bioscience reports · 2022Article
- Hypoxia controls plasma membrane targeting of polarity proteins by dynamic turnover of PI4P and PI(4,5)P2.eLife · 2022Article
- Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.Journal of cell science · 2021Article
- Article
- FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation.Journal of visualized experiments : JoVE · 2018Article
- Notum coordinates synapse development via extracellular regulation of Wingless trans-synaptic signaling.Development (Cambridge, England) · 2017Article
- Secreted tissue inhibitor of matrix metalloproteinase restrictsJournal of cell science · 2017Article
- Downregulation of RBO-PI4KIIIα Facilitates AβThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis.PLoS biology · 2017Article
- Article
- Using theFrontiers in pediatrics · 2017Review
- EFR3s are palmitoylated plasma membrane proteins that control responsiveness to G-protein-coupled receptors.Journal of cell science · 2015Article
- Expression of EFR3A in the mouse cochlea during degeneration of spiral ganglion following hair cell loss.PloS one · 2015Article
- The cell polarity scaffold Lethal Giant Larvae regulates synapse morphology and function.Journal of cell science · 2013Article
- Regulation of synaptic vesicle budding and dynamin function by an EHD ATPase.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The Role of GSK3 in Presynaptic Function.International journal of Alzheimer's disease · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rolling blackout (RBO) is a Drosophila EFR3 integral membrane lipase. A conditional temperature-sensitive (TS) mutant (rbo(ts)) displays paralysis within minutes following a temperature shift from 25 degrees C to 37 degrees C, an impairment previously attributed solely to blocked synaptic-vesicle exocytosis. However, we found that rbo(ts) displays a strong synergistic interaction with the Syntaxin-1A TS allele syx(3-69), recently shown to be a dominant positive mutant that increases Syntaxin-1A function. At neuromuscular synapses, rbo(ts) showed a strong defect in styryl-FM-dye (FM) endocytosis, and rbo(ts);syx(3-69) double mutants displayed a synergistic, more severe, endocytosis impairment. Similarly, central rbo(ts) synapses in primary brain culture showed severely defective FM endocytosis. Non-neuronal nephrocyte Garland cells showed the same endocytosis defect in tracer-uptake assays. Ultrastructurally, rbo(ts) displayed a specific defect in tracer uptake into endosomes in both neuronal and non-neuronal cells. At the rbo(ts) synapse, there was a total blockade of endosome formation via activity-dependent bulk endocytosis. Clathrin-mediated endocytosis was not affected; indeed, there was a significant increase in direct vesicle formation. Together, these results demonstrate that RBO is required for constitutive and/or bulk endocytosis and/or macropinocytosis in both neuronal and non-neuronal cells, and that, at the synapse, this mechanism is responsive to the rate of Syntaxin-1A-dependent exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.