ArticleSexually transmitted infections2009
Evaluation of multiplex real-time PCR for detection of Haemophilus ducreyi, Treponema pallidum, herpes simplex virus type 1 and 2 in the diagnosis of genital ulcer disease in the Rakai District, Uganda.
Article in Sexually transmitted infections, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
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Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 69 citations in OpenAlex.
- Pooled it
- Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis.Journal of clinical microbiology · 2015Pooled it
- Etiology of genital ulcer disease and association with HIV infection in Malawi.Sexually transmitted diseases · 2013Trial
- Reactivation of herpes simplex virus type 2 after initiation of antiretroviral therapy.The Journal of infectious diseases · 2013Trial
- Aetiology of genital ulcer disease in female partners of male participants in a circumcision trial in Uganda.International journal of STD & AIDS · 2009Trial
- Infections caused byClinical microbiology reviews · 2024Review
- Molecular Characterization of Genital and Extragenital Lesions With the PlexPCR VHS Assay in Patients Diagnosed With Syphilis.Open forum infectious diseases · 2023Article
- British Columbia's Experience after Implementation of the Treponema pallidum Reverse Algorithm and PCR Detection, 2015 to 2020.Microbiology spectrum · 2022Article
- Polymerase chain reaction for the diagnosis of herpesvirus infections in dermatology : Analysis of clinical data.Wiener klinische Wochenschrift · 2020Article
- Microbial Diversity of Genital Ulcers of HSV-2 Seropositive Women.Scientific reports · 2017Article
- Use of injectable hormonal contraception and women's risk of herpes simplex virus type 2 acquisition: a prospective study of couples in Rakai, Uganda.The Lancet. Global health · 2015Article
- Comparative performance of the Uni-Gold™ HSV-2 Rapid: a point-of-care HSV-2 diagnostic test in unselected sera from a reference laboratory.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2014Article
- Financial implications of male circumcision scale-up for the prevention of HIV and other sexually transmitted infections in a sub-Saharan African community.Sexually transmitted diseases · 2013Article
- Herpes simplex virus type-2 assay specificity and male circumcision to reduce herpes simplex virus type-2 acquisition.AIDS (London, England) · 2013Article
- A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections.Biochip journal · 2013Article
- Etiology of genital ulcer disease in a sexually transmitted infection reference center in Manaus, Brazilian Amazon.PloS one · 2013Article
- Risk factors, healthcare-seeking and sexual behaviour among patients with genital ulcers in Zambia.BMC public health · 2012Article
- Herpes diagnostic tests and their use.Current infectious disease reports · 2012Article
- Passive immunization with a polyclonal antiserum to the hemoglobin receptor of Haemophilus ducreyi confers protection against a homologous challenge in the experimental swine model of chancroid.Infection and immunity · 2011Article
- HIV and other sexually transmitted infections in a cohort of women involved in high-risk sexual behavior in Kampala, Uganda.Sexually transmitted diseases · 2011Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveTo develop a real-time PCR assay that reliably and accurately detects the predominant sexually transmitted aetiological agents of genital ulcer disease (GUD) (Haemophilus ducreyi, Treponema pallidum and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2)) and to assess the use of real-time PCR diagnostic testing in a rural African field site.
methodsTwo multiplex real-time PCR reactions were used to detect H ducreyi/and HSV-1/HSV-2 in ulcer swabs from 100 people with symptomatic genital ulcers in rural Rakai, Uganda. Results were compared with syphilis, HSV-1 and HSV-2 serology.
resultsOf 100 GUD samples analysed from 43 HIV positive and 57 HIV negative individuals, 71% were positive for one or more sexually transmitted infection (STI) pathogens by real-time PCR (61% for HSV-2, 5% for T pallidum, 3% for HSV-1, 1% for H ducreyi and 1% for dual H ducreyi/HSV-2). The frequency of HSV in genital ulcers was 56% (32/57) in HIV negative individuals and 77% (33/43) in HIV positive individuals (p = 0.037). Assay reproducibility was evaluated by repeat PCR testing in the USA with 96% agreement (kappa = 0.85).
conclusionsSTI pathogens were detected in the majority of GUD swab samples from symptomatic patients in Rakai, Uganda, by real-time PCR. HSV-2 was the predominant cause of genital ulcers. Real-time PCR technology can provide sensitive, rapid and reproducible evaluation of GUD aetiology in a resource-limited setting.
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