ArticleProceedings of the National Academy of Sciences of the United States of America2008
Dynein pulls microtubules without rotating its stalk.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Versatile properties of dynein molecules underlying regulation in flagellar oscillation.Scientific reports · 2023Article
- ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.The EMBO journal · 2023Article
- Article
- Structure of Motile Cilia.Sub-cellular biochemistry · 2022Article
- Article
- Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble.International journal of molecular sciences · 2020Review
- Sliding Mechanism at a Coiled-Coil Interface.Biophysical journal · 2019Article
- Step Sizes and Rate Constants of Single-headed Cytoplasmic Dynein Measured with Optical Tweezers.Scientific reports · 2018Article
- Turning dyneins off bends cilia.Cytoskeleton (Hoboken, N.J.) · 2018Article
- A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1).Molecular biology of the cell · 2018Article
- Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules.Nature communications · 2015Article
- Dynein-deficient flagella respond to increased viscosity with contrasting changes in power and recovery strokes.Cytoskeleton (Hoboken, N.J.) · 2015Article
- The winch model can explain both coordinated and uncoordinated stepping of cytoplasmic dynein.Biophysical journal · 2014Article
- Structural mechanism of the dynein power stroke.Nature cell biology · 2014Article
- Functions and mechanics of dynein motor proteins.Nature reviews. Molecular cell biology · 2013Review
- Big steps toward understanding dynein.The Journal of cell biology · 2013Review
- Article
- 3D structure of eukaryotic flagella/cilia by cryo-electron tomography.Biophysics (Nagoya-shi, Japan) · 2013Review
- ATP-driven remodeling of the linker domain in the dynein motor.Structure (London, England : 1993) · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynein is a microtubule motor that powers motility of cilia and flagella. There is evidence that the relative sliding of the doublet microtubules is due to a conformational change in the motor domain that moves a microtubule bound to the end of an extension known as the stalk. A predominant model for the movement involves a rotation of the head domain, with its stalk, toward the microtubule plus end. However, stalks bound to microtubules have been difficult to observe. Here, we present the clearest views so far of stalks in action, by observing sea urchin, outer arm dynein molecules bound to microtubules, with a new method, "cryo-positive stain" electron microscopy. The dynein molecules in the complex were shown to be active in in vitro motility assays. Analysis of the electron micrographs shows that the stalk angles relative to microtubules do not change significantly between the ADP.vanadate and no-nucleotide states, but the heads, together with their stalks, shift with respect to their A-tubule attachments. Our results disagree with models in which the stalk acts as a lever arm to amplify structural changes. The observed movement of the head and stalk relative to the tail indicates a new plausible mechanism, in which dynein uses its stalk as a grappling hook, catching a tubulin subunit 8 nm ahead and pulling on it by retracting a part of the tail (linker).
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