ArticleProceedings of the National Academy of Sciences of the United States of America2008
Expanding metabolism for biosynthesis of nonnatural alcohols.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
- Article
- EngineeringBiodesign research · 2025Article
- Computational tools for nonnatural pathway design: Algorithms, applications, and challenges.Biodesign research · 2025Review
- Directed evolution of α-ketoisovalerate decarboxylase for improved isobutanol and 3-methyl-1-butanol production in cyanobacteria.Biotechnology for biofuels and bioproducts · 2025Article
- Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism.Nature communications · 2025Article
- Mutualism breakdown underpins evolutionary rescue in an obligate cross-feeding bacterial consortium.Nature communications · 2025Article
- Production of (R)-citramalate by engineeredMetabolic engineering communications · 2024Article
- Characterization of HphA: The First Enzyme in the Homologation Pathway of l-Phenylalanine and l-Tyrosine.Chembiochem : a European journal of chemical biology · 2024Article
- Concentration Recognition-Based Auto-Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. coli from inexpensive aromatic precursors.Microbial cell factories · 2024Article
- A Zero-Valent Sulfur Transporter Helps Podophyllotoxin Uptake into Bacterial Cells in the Presence of CTAB.Antioxidants (Basel, Switzerland) · 2023Article
- Escherichia coli displays a conserved membrane proteomic response to a range of alcohols.Biotechnology for biofuels and bioproducts · 2023Article
- A novel strategy for L-arginine production in engineered Escherichia coli.Microbial cell factories · 2023Article
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- Advances in biosynthesis of higher alcohols in Escherichia coli.World journal of microbiology & biotechnology · 2023Review
- The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae.Biotechnology for biofuels and bioproducts · 2022Article
- ROCK inhibitors modulate the physical properties and adipogenesis of 3D spheroids of human orbital fibroblasts in different manners.FASEB bioAdvances · 2021Article
- Metabolic energy conservation for fermentative product formation.Microbial biotechnology · 2021Review
- A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine inFrontiers in bioengineering and biotechnology · 2021Article
- Coupled Electrochemical and Microbial Catalysis for the Production of Polymer Bricks.ChemSusChem · 2020Article
41 more citing papers are in PubMed but not listed here.
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Nature uses a limited set of metabolites to perform all of the biochemical reactions. To increase the metabolic capabilities of biological systems, we have expanded the natural metabolic network, using a nonnatural metabolic engineering approach. The branched-chain amino acid pathways are extended to produce abiotic longer chain keto acids and alcohols by engineering the chain elongation activity of 2-isopropylmalate synthase and altering the substrate specificity of downstream enzymes through rational protein design. When introduced into Escherichia coli, this nonnatural biosynthetic pathway produces various long-chain alcohols with carbon number ranging from 5 to 8. In particular, we demonstrate the feasibility of this approach by optimizing the biosynthesis of the 6-carbon alcohol, (S)-3-methyl-1-pentanol. This work demonstrates an approach to build artificial metabolism beyond the natural metabolic network. Nonnatural metabolites such as long chain alcohols are now included in the metabolite family of living systems.
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