ArticleToxicological sciences : an official journal of the Society of Toxicology2009
Werner syndrome protein, WRN, protects cells from DNA damage induced by the benzene metabolite hydroquinone.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Genetic variations in ATM and H2AX loci contribute to risk of hematological abnormalities in individuals exposed to BTEX chemicals.Journal of clinical laboratory analysis · 2022Article
- Different non-synonymous polymorphisms modulate the interaction of the WRN protein to its protein partners and its enzymatic activities.Oncotarget · 2016Article
- Identification of Genes That Modulate Susceptibility to Formaldehyde and Imatinib by Functional Genomic Screening in Human Haploid KBM7 Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Potential relationship between inadequate response to DNA damage and development of myelodysplastic syndrome.International journal of molecular sciences · 2015Review
- Emerging approaches in predictive toxicology.Environmental and molecular mutagenesis · 2014Article
- Application of toxicogenomic profiling to evaluate effects of benzene and formaldehyde: from yeast to human.Annals of the New York Academy of Sciences · 2014Review
- Current understanding of the mechanism of benzene-induced leukemia in humans: implications for risk assessment.Carcinogenesis · 2012Review
- Comparison of proliferation and genomic instability responses to WRN silencing in hematopoietic HL60 and TK6 cells.PloS one · 2011Article
- Genome-wide functional profiling reveals genes required for tolerance to benzene metabolites in yeast.PloS one · 2011Article
- Systems biology of human benzene exposure.Chemico-biological interactions · 2010Review
- Roles of Werner syndrome protein in protection of genome integrity.DNA repair · 2010Review
- Advances in understanding benzene health effects and susceptibility.Annual review of public health · 2010Review
- Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9.Mutation research. Reviews in mutation researchReview
- Cellular deficiency of Werner syndrome protein or RECQ1 promotes genotoxic potential of hydroquinone and benzo[a]pyrene exposure.International journal of toxicologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Werner syndrome (WS) is a rare autosomal progeroid disorder caused by a mutation in the gene encoding the WRN (Werner syndrome protein), a member of the RecQ family of helicases with a role in maintaining genomic stability. Genetic association studies have previously suggested a link between WRN and susceptibility to benzene-induced hematotoxicity. To further explore the role of WRN in benzene-induced hematotoxicity, we used short hairpin RNA to silence endogenous levels of WRN in the human HL60 acute promyelocytic cell line and subsequently exposed the cells to hydroquinone (HQ). Suppression of WRN led to an accelerated cell growth rate, increased susceptibility to hydroquinone-induced cytotoxicity and genotoxicity as measured by the single-cell gel electrophoresis assay, and an enhanced DNA damage response. More specifically, loss of WRN resulted in higher levels of early apoptosis, marked by increases in relative levels of cleaved caspase-7 and cleaved poly (ADP-ribose) polymerase 1, in cells treated with HQ compared with control cells. Our data suggests that WRN plays an important role in the surveillance of and protection against DNA damage induced by HQ. This provides mechanistic support for the link between WRN and benzene-induced hematotoxicity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.