ArticleMolecular and cellular biology2009
Spliceosome assembly pathways for different types of alternative splicing converge during commitment to splice site pairing in the A complex.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- REAL-TIME VISUALIZATION OF SPLICEOSOME ASSEMBLY REVEALS BASIC PRINCIPLES OF 3' SPLICE SITE SELECTION.Research square · 2026Article
- RNA splicing: a split consensus reveals two major 5' splice site classes.Open biology · 2025Review
- Exon-independent recruitment of SRSF1 is mediated by U1 snRNP stem-loop 3.The EMBO journal · 2022Article
- Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin β1.International journal of oncology · 2017Article
- Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.Nucleic acids research · 2017Article
- Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.Methods in molecular biology (Clifton, N.J.) · 2014Article
- In vitro assay of pre-mRNA splicing in mammalian nuclear extract.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy.Cell reports · 2013Article
- Splicing kinetics and transcript release from the chromatin compartment limit the rate of Lipid A-induced gene expression.RNA (New York, N.Y.) · 2013Article
- Pick one, but be quick: 5' splice sites and the problems of too many choices.Genes & development · 2013Review
- Position-dependent splicing activation and repression by SR and hnRNP proteins rely on common mechanisms.RNA (New York, N.Y.) · 2013Article
- The transition in spliceosome assembly from complex E to complex A purges surplus U1 snRNPs from alternative splice sites.Nucleic acids research · 2012Article
- The spliceosome: a flexible, reversible macromolecular machine.Trends in biochemical sciences · 2012Review
- U1 snRNA directly interacts with polypyrimidine tract-binding protein during splicing repression.Molecular cell · 2011Article
- Retention of spliceosomal components along ligated exons ensures efficient removal of multiple introns.RNA (New York, N.Y.) · 2010Article
- First come, first served revisited: factors affecting the same alternative splicing event have different effects on the relative rates of intron removal.RNA (New York, N.Y.) · 2010Article
- Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches.Nature reviews. Molecular cell biology · 2009Review
- Dangerous play--splitting the message may leave you empty handed.Nature structural & molecular biology · 2009Article
- Differential 3' splice site recognition of SMN1 and SMN2 transcripts by U2AF and U2 snRNP.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Differential splice site pairing establishes alternative splicing patterns resulting in the generation of multiple mRNA isoforms. This process is carried out by the spliceosome, which is activated by a series of sequential structural rearrangements of its five core snRNPs. To determine when splice sites become functionally paired, we carried out a series of kinetic trap experiments using pre-mRNAs that undergo alternative 5' splice site selection or alternative exon inclusion. We show that commitment to splice site pairing in both cases occurs in the A complex, which is characterized by the ATP-dependent association of the U2 snRNP with the branch point. Interestingly, the timing of splice site pairing is independent of the intron or exon definition modes of splice site recognition. Using the ATP analog ATPgammaS, we showed that ATP hydrolysis is required for splice site pairing independent from U2 snRNP binding to the pre-mRNA. These results identify the A complex as the spliceosomal assembly step dedicated to splice site pairing and suggest that ATP hydrolysis locks splice sites into a splicing pattern after stable U2 snRNP association to the branch point.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.