ArticleJournal of structural biology2009
Organization of human replicon: singles or zipping couples?
Article in Journal of structural biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- The kinetics of uracil-N-glycosylase distribution inside replication foci.Scientific reports · 2025Article
- Mechanisms of tandem duplication in the cancer genome.DNA repair · 2025Review
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- Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis.International journal of molecular sciences · 2021Review
- The Safe Path at the Fork: Ensuring Replication-Associated DNA Double-Strand Breaks are Repaired by Homologous Recombination.Frontiers in genetics · 2021Review
- Article
- DNA Replication: From Radioisotopes to Click Chemistry.Molecules (Basel, Switzerland) · 2018Review
- A Simple Marker-Assisted 3D Nanometer Drift Correction Method for Superresolution Microscopy.Biophysical journal · 2017Article
- A journey through the microscopic ages of DNA replication.Protoplasma · 2017Review
- Article
- Article
- 4D Visualization of replication foci in mammalian cells corresponding to individual replicons.Nature communications · 2016Article
- Article
- Stochastic association of neighboring replicons creates replication factories in budding yeast.The Journal of cell biology · 2013Article
- Genome-wide copy number profiling of single cells in S-phase reveals DNA-replication domains.Nucleic acids research · 2013Article
- Bringing single-molecule spectroscopy to macromolecular protein complexes.Trends in biochemical sciences · 2013Review
- Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ.PloS one · 2012Article
- MCM2-7 form double hexamers at licensed origins in Xenopus egg extract.The Journal of biological chemistry · 2011Article
- Uncoupling of sister replisomes during eukaryotic DNA replication.Molecular cell · 2010Article
- Spatial regulation and organization of DNA replication within the nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
According to a general paradigm, proper DNA duplication from each replication origin is ensured by two protein complexes termed replisomes. In prokaryotes and in budding yeast Saccharomyces cerevisiae, these two replisomes seem to be associated with one another until DNA replication initiated from the origin has finished. This arrangement results in the formation of the loop of newly synthesized DNA. However, arrangement of replisomes in other eukaryotic organisms including vertebrate cells is largely unknown. Here, we used in vivo labeling of DNA segments in combination with the electron microscopy tomography to describe the organization of replisomes in human HeLa cells. The experiments were devised in order to distinguish between a model of independent replisomes and a model of replisome couples. The comparative analysis of short segments of replicons labeled in pulse-chase experiments of various length shows that replisomes in HeLa cells are organized into the couples during DNA replication. Moreover, our data enabled to suggest a new model of the organization of replicated DNA. According to this model, replisome couples produce loop with the associated arms in the form of four tightly associated 30nm fibers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.