ArticleBiochemical and biophysical research communications2009
Feedback regulation of cyclooxygenase-2 transcription ex vivo and in vivo.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Glycosylated Delphinidins, Natural Antioxidants, Functionally Modulate the COX-2/PGEAntioxidants (Basel, Switzerland) · 2026Article
- Prostaglandins from Cytosolic Phospholipase A2α/Cyclooxygenase-1 Pathway and Mitogen-activated Protein Kinases Regulate Gene Expression in Candida albicans-infected Macrophages.The Journal of biological chemistry · 2016Article
- Candidate chromosome 1 disease susceptibility genes for Sjogren's syndrome xerostomia are narrowed by novel NOD.B10 congenic mice.Clinical immunology (Orlando, Fla.) · 2014Article
- Molecular docking and analgesic studies of Erythrina variegata׳s derived phytochemicals with COX enzymes.Bioinformation · 2014Article
- Low doses of celecoxib attenuate gut barrier failure during experimental peritonitis.Laboratory investigation; a journal of technical methods and pathology · 2013Article
- Article
- In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals.Sensors (Basel, Switzerland) · 2011Review
- Evidence for a spatial and temporal regulation of prostaglandin-endoperoxide synthase 2 expression in human amnion in term and preterm parturition.The Journal of clinical endocrinology and metabolism · 2010Article
- Cox-2 gene expression in chemically induced skin papillomas cannot predict subsequent tumor fate.Molecular oncology · 2010Article
- Molecular imaging of transcriptional regulation during inflammation.Journal of inflammation (London, England) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cyclooxygenase-2 (COX-2) is a rate-limiting enzyme for prostaglandin biosynthesis. Its inducible expression is regulated by complex pathways. To monitor Cox-2 transcriptional activity in vivo, we generated a knock-in mouse expressing a firefly luciferase reporter. In this study we examined, by comparing luciferase activity of Cox-2(luc/+) and Cox-2(luc/-) cells and mice, effects of prostanoid products on Cox-2 promoter transcriptional activation. In peritoneal macrophages, luciferase induction by LPS in Cox-2(luc/-) cells was less than that of Cox-2(luc/+) cells. However, in the presence of PGE(2), induction was comparable, suggesting positive Cox-2 feedback regulation by PGE(2) occurs for macrophages. In contrast, feedback modulation was not observed in TPA-induced Cox-2(luc/+) and Cox-2(luc/-) mouse embryonic fibroblasts (MEFs). Using non-invasive in vivo imaging, we observed negative feedback regulation of Cox-2 expression during paw inflammation in living mice. Our results suggest Cox-2 expression is regulated by cell type specific feedback mechanisms, both in cultured cells and in living animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.