ArticleMolecular cell2008
Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Cryo-EM study of bacteriophage N4 virion RNA polymerase.Journal of bacteriology · 2026Article
- Structural choreography of bacteriophage N4 ejection proteins and the giant virion-associated RNA polymerase.Nature communications · 2026Article
- The -10 region adjacent to open reading frames is a common expression pattern inLife science alliance · 2025Article
- Structure of the giant RNA polymerase ejected from coliphage N4.Research square · 2025Article
- A comparative study of protein-ssDNA interactions.NAR genomics and bioinformatics · 2021Article
- Article
- Global Proteomic Profiling ofJournal of virology · 2019Article
- Minimalism and functionality: Structural lessons from the heterodimeric N4 bacteriophage RNA polymerase II.The Journal of biological chemistry · 2018Article
- A DNA structural alphabet provides new insight into DNA flexibility.Acta crystallographica. Section D, Structural biology · 2018Article
- Article
- To Be or Not To Be T4: Evidence of a Complex Evolutionary Pathway of Head Structure and Assembly in GiantFrontiers in microbiology · 2017Article
- Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases.Biomolecules · 2015Review
- A model for transcription initiation in human mitochondria.Nucleic acids research · 2015Article
- Development of giant bacteriophage ϕKZ is independent of the host transcription apparatus.Journal of virology · 2014Article
- A novel intermediate in transcription initiation by human mitochondrial RNA polymerase.Nucleic acids research · 2014Article
- Structure of Escherichia coli RNA polymerase holoenzyme at last.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- NusG-Spt5 proteins-Universal tools for transcription modification and communication.Chemical reviews · 2013Review
- Biochemical analysis of histone deacetylase-independent transcriptional repression by MeCP2.The Journal of biological chemistry · 2013Article
- Highly similar structural frames link the template tunnel and NTP entry tunnel to the exterior surface in RNA-dependent RNA polymerases.Nucleic acids research · 2013Article
- Watching the bacteriophage N4 RNA polymerase transcription by time-dependent soak-trigger-freeze X-ray crystallography.The Journal of biological chemistry · 2013Article
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Authors and funding
4 authors.
Funding
Abstract
Coliphage N4 virion-encapsidated RNA polymerase (vRNAP) is a member of the phage T7-like single-subunit RNA polymerase (RNAP) family. Its central domain (mini-vRNAP) contains all RNAP functions of the full-length vRNAP, which recognizes a 5 to 7 base pair stem and 3 nucleotide loop hairpin DNA promoter. Here, we report the X-ray crystal structures of mini-vRNAP bound to promoters. Mini-vRNAP uses four structural motifs to recognize DNA sequences at the hairpin loop and stem and to unwind DNA. Despite their low sequence similarity, three out of four motifs are shared with T7 RNAP that recognizes a double-stranded DNA promoter. The binary complex structure and results of engineered disulfide linkage experiments reveal that the plug and motif B loop, which block the access of template DNA to the active site in the apo-form mini-vRNAP, undergo a large-scale conformational change upon promoter binding, explaining the restricted promoter specificity that is critical for N4 phage early transcription.
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