ArticleMolecular cell2008
Multimegabase silencing in nucleolar dominance involves siRNA-directed DNA methylation and specific methylcytosine-binding proteins.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 144 citations in OpenAlex.
- Gene regulatory mechanisms downstream of DNA methylation.Nature reviews. Genetics · 2026Review
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Characterization of DNA methylation reader proteins inGenome research · 2024Article
- Transcriptional Silencing of 35S rDNA inInternational journal of molecular sciences · 2024Article
- The MBD-ACD DNA methylation reader complex recruits MICRORCHIDIA6 to regulate ribosomal RNA gene expression in Arabidopsis.The Plant cell · 2024Article
- Functions of RNAi Pathways in Ribosomal RNA Regulation.Non-coding RNA · 2024Review
- The chromatin source-sink hypothesis: a shared mode of chromatin-mediated regulations.Development (Cambridge, England) · 2023Article
- A GFP splicing reporter in a coilin mutant background reveals links between alternative splicing, siRNAs, and coilin function in Arabidopsis thaliana.G3 (Bethesda, Md.) · 2023Article
- Reaching for the off switch in nucleolar dominance.The Plant journal : for cell and molecular biology · 2023Article
- Enzymatic reactions of AGO4 in RNA-directed DNA methylation: siRNA duplex loading, passenger strand elimination, target RNA slicing, and sliced target retention.Genes & development · 2023Article
- Transcriptional multiomics reveals the mechanism of seed deterioration in Nicotiana tabacum L. and Oryza sativa L.Journal of advanced research · 2022Article
- Genomic architecture of 5S rDNA cluster and its variations within and between species.BMC genomics · 2022Article
- Tracing the Evolution of the Angiosperm Genome from the Cytogenetic Point of View.Plants (Basel, Switzerland) · 2022Review
- Structure and Methylation of 35S rDNA in AllopolyploidsFrontiers in plant science · 2022Article
- Wheat Breeding, Transcription Factories, and Genetic Interactions: New Perspectives.Frontiers in plant science · 2022Article
- The integrative biology of genetic dominance.Biological reviews of the Cambridge Philosophical Society · 2021Review
- An essential role for the piRNA pathway in regulating the ribosomal RNA pool in C. elegans.Developmental cell · 2021Article
- MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO.Science (New York, N.Y.) · 2021Article
- Biological role and mechanism of chromatin readers in plants.Current opinion in plant biology · 2021Review
28 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
In genetic hybrids, the silencing of nucleolar rRNA genes inherited from one progenitor is the epigenetic phenomenon known as nucleolar dominance. An RNAi knockdown screen identified the Arabidopsis de novo cytosine methyltransferase, DRM2, and the methylcytosine binding domain proteins, MBD6 and MBD10, as activities required for nucleolar dominance. MBD10 localizes throughout the nucleus, but MBD6 preferentially associates with silenced rRNA genes and does so in a DRM2-dependent manner. DRM2 methylation is thought to be guided by siRNAs whose biogenesis requires RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE 3 (DCL3). Consistent with this hypothesis, knockdown of DCL3 or RDR2 disrupts nucleolar dominance. Collectively, these results indicate that in addition to directing the silencing of retrotransposons and noncoding repeats, siRNAs specify de novo cytosine methylation patterns that are recognized by MBD6 and MBD10 in the large-scale silencing of rRNA gene loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.