ArticleAnalytical chemistry2009
Direct observation of conformation of a polymeric coating with implications in microarray applications.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development.Biosensors · 2025Article
- Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays.Biosensors · 2024Article
- Optimization of Functional Group Concentration of N, N-Dimethylacrylamide-based Polymeric Coatings and Probe Immobilization for DNA and Protein Microarray Applications.Micromachines · 2023Article
- Attomolar sensitivity microRNA detection using real-time digital microarrays.Scientific reports · 2022Article
- The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors.Polymers · 2022Article
- The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview.Polymers · 2021Review
- Non-Langmuir Kinetics of DNA Surface Hybridization.Biophysical journal · 2020Article
- Review
- Multispot, label-free biodetection at a phantom plastic-water interface.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- TATA binding proteins can recognize nontraditional DNA sequences.Biophysical journal · 2012Article
- Multiplexed method to calibrate and quantitate fluorescence signal for allergen-specific IgE.Analytical chemistry · 2011Article
- Label-free multiplexed virus detection using spectral reflectance imaging.Biosensors & bioelectronics · 2011Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- Multiplexed, rapid point of care platform to quantify allergen-specific IgE.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2011Article
- Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches.Biosensors & bioelectronics · 2010Article
- Quantification of DNA and protein adsorption by optical phase shift.Biosensors & bioelectronics · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The conformation of a three-dimensional polymeric coating (copoly(DMA-NAS-MAPS)) and immobilization and hybridization of DNA strands on the polymer coated surface are investigated. A conformational change, specifically the swelling of the surface adsorbed polymer upon hydration, is quantified in conjunction with the application of this polymer coating for DNA microarray applications. Fluorescently labeled short DNA strands (23mers) covalently linked to the functional groups on the adsorbed polymer are used as probes to measure the swelling of the polymer. A fluorescence microscopy technique, Spectral Self-Interference Fluorescence Microscopy (SSFM), is utilized to directly measure the change in axial position of fluorophores due to swelling with subnanometer accuracy. Additionally, immobilization characteristics of single stranded DNA (ssDNA) and double stranded DNA (dsDNA) probes, as well as hybridization of ssDNA with target strands have been studied. The results show that ssDNA further away from the surface is hybridized more efficiently, which strengthens the earlier analysis of this polymeric coating as a simple but highly efficient and robust DNA microarray surface chemistry.
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Registered trials
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