ArticleBiomacromolecules2009
Analysis of oligonucleotide DNA binding and sedimentation properties of montmorillonite clay using ultraviolet light spectroscopy.
Article in Biomacromolecules, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Bentonite-Clarified White Wine: Linking Clay Physico-Chemical Properties to Protein Removal Efficiency and Wine Matrix Alterations.Molecules (Basel, Switzerland) · 2025Article
- Therapeutic Oligonucleotides for Neurodegenerative Diseases: Aptamer Strategies and Clay Nanoparticle-Based Delivery.Chemical record (New York, N.Y.) · 2025Review
- Soil Phosphorus Dynamics are an Overlooked but Dominant Control on Mineral-Associated Organic Matter.Global change biology · 2025Article
- Spectrophotometric and nucleic acid-binding properties of halloysite clay nanotubes and kaolinite.Heliyon · 2023Article
- RIAM: A Universal Accessible Protocol for the Isolation of High Purity DNA from Various Soils and Other Humic Substances.Methods and protocols · 2022Article
- Clays as Vehicles for Drug Photostability.Pharmaceutics · 2022Review
- Fifteen shades of clay: distinct microbial community profiles obtained from bentonite samples by cultivation and direct nucleic acid extraction.Scientific reports · 2021Article
- Infrared Nanospectroscopy Reveals DNA Structural Modifications upon Immobilization onto Clay Nanotubes.Nanomaterials (Basel, Switzerland) · 2021Article
- pH-dependent sedimentation of DNA in the presence of divalent, but not monovalent, metal ions.Analytical biochemistry · 2021Article
- Facile Fabrication of Natural Polyelectrolyte-Nanoclay Composites: Halloysite Nanotubes, Nucleotides and DNA Study.Molecules (Basel, Switzerland) · 2020Article
- Preliminary evidences of the presence of extracellular DNA single stranded forms in soil.PloS one · 2020Article
- Validating DNA Extraction Protocols for Bentonite Clay.mSphere · 2019Article
- Factors affecting the association of single- and double-stranded RNAs with montmorillonite nanoclays.International journal of biological macromolecules · 2018Article
- Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells.Scientific reports · 2016Article
- Impact of size, secondary structure, and counterions on the binding of small ribonucleic acids to layered double hydroxide nanoparticles.Biointerphases · 2015Article
- Charge density and particle size effects on oligonucleotide and plasmid DNA binding to nanosized hydrotalcite.Biointerphases · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Smectite clays such as montmorillonite form complexes with a variety of biomolecules, including the nucleic acids DNA and RNA. Most previous studies of DNA adsorption onto clay have relied on spectrophotometric analysis after the separation of free nucleic acids from bound complexes by centrifugation. In the current work, we demonstrate that such studies produce a consistent error because of (a) incomplete sedimentation of montmorillonite and (b) strong absorbance of the remaining clay at 260 nm. Clay sedimentation efficiency was strongly dependent on cation concentration (Na+ or Mg2+) and on the level of dispersion of the original suspension. An improved clay-DNA adsorption assay was developed and utilized to assess the impact of metal counterions on the binding of single-stranded DNA to montmorillonite. X-ray diffraction demonstrated, for the first time, the formation of intercalated structures consistent with orientation of the DNA strands parallel to the clay surface. Observed gallery spacings were found to match values calculated using atomistic modeling techniques closely.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.