ArticleProceedings of the National Academy of Sciences of the United States of America2008
Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- Article
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- Ketamine induces plasticity in a norepinephrine-astroglial circuit to promote behavioral perseverance.Neuron · 2025Article
- Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish (Zoological research · 2024Article
- LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition.Developmental biology · 2023Article
- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
- Article
- Zebrafish Is a Powerful Tool for Precision Medicine Approaches to Neurological Disorders.Frontiers in molecular neuroscience · 2022Review
- Proof of principle for piggyBac-mediated transgenesis in the flatworm Macrostomum lignano.Genetics · 2021Article
- Enhancer Trapping and Annotation in Zebrafish Mediated with Sleeping Beauty, piggyBac and Tol2 Transposons.Genes · 2018Article
- Brain-wide Organization of Neuronal Activity and Convergent Sensorimotor Transformations in Larval Zebrafish.Neuron · 2018Article
- Color opponency with a single kind of bistable opsin in the zebrafish pineal organ.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.Nucleic acids research · 2018Article
- Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors.Journal of visualized experiments : JoVE · 2018Article
- Preclinical and clinical advances in transposon-based gene therapy.Bioscience reports · 2017Review
- Imaging Subcellular Structures in the Living Zebrafish Embryo.Journal of visualized experiments : JoVE · 2016Article
- A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types.eLife · 2016Article
- Identification of environmental chemicals that induce yolk malabsorption in zebrafish using automated image segmentation.Reproductive toxicology (Elmsford, N.Y.) · 2015Article
- Mosaic zebrafish transgenesis for functional genomic analysis of candidate cooperative genes in tumor pathogenesis.Journal of visualized experiments : JoVE · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Tol2 transposable element is a powerful genetic tool in model vertebrates and has been used for transgenesis, insertional mutagenesis, gene trapping, and enhancer trapping. However, an in vivo transposition system using Tol2 has not yet been developed. Here we report the in vivo Tol2 transposition system in a model vertebrate, zebrafish. First, we constructed transgenic zebrafish that carried single-copy integrations of Tol2 on the genome and injected transposase mRNA into one-cell stage embryos. The Tol2 insertions were mobilized efficiently in the germ lineage. We then mobilized an insertion of the Tol2 gene trap construct in the nup214 gene, which caused a recessive lethal mutant phenotype, and demonstrated that this method is applicable to the isolation of revertants from a transposon insertional mutant. Second, we constructed transgenic fish carrying the transposase cDNA under the control of the hsp70 promoter. Double-transgenic fish containing the transposase gene and a single-copy Tol2 insertion were treated with heat shock at the adult stage. We found that transposition can be induced efficiently in the male germ cells. We analyzed new integration sites and found that the majority (83%) of them were mapped on chromosomes other than the transposon donor chromosomes and that 9% of local hopping events mapped less than 300 kb away from the donor loci. Our present study demonstrates that the in vivo Tol2 transposition system is useful for creating genome-wide insertions from a single-copy donor and should facilitate functional genomics and transposon biology in vertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.