ArticleApplied and environmental microbiology2009
Energy metabolism response to low-temperature and frozen conditions in Psychrobacter cryohalolentis.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- How long do pathogens persist and survive in water? A systematic review.Frontiers in microbiology · 2025Pooled it
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- Investigation of cold-resistance mechanisms in cryophylactic yeastFrontiers in microbiology · 2024Article
- The Cytotoxic Properties of Extreme Fungi's Bioactive Components-An Updated Metabolic and Omics Overview.Life (Basel, Switzerland) · 2023Review
- Thermal acclimation of methanotrophs from the genus Methylobacter.The ISME journal · 2023Article
- Diversity of the protease-producing bacteria and their extracellular protease in the coastal mudflat of Jiaozhou Bay, China: in response to clam naturally growing and aquaculture.Frontiers in microbiology · 2023Article
- Genomic and phenotypic characterization of a red-pigmented strain ofFrontiers in microbiology · 2023Article
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- Microbial ecology of the atmosphere.FEMS microbiology reviews · 2022Review
- Investigating the Functional Role of Hypothetical Proteins From an Antarctic BacteriumFrontiers in genetics · 2022Article
- The cold adaption profiles ofBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production.Scientific reports · 2021Article
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Five animal phyla in glacier ice reveal unprecedented biodiversity in New Zealand's Southern Alps.Scientific reports · 2021Article
- Changes in the Concentration of Purine and Pyridine as a Response to Single Whole-Body Cryostimulation.Frontiers in physiology · 2021Article
- A comparative UHPLC-Q/TOF-MS-based eco-metabolomics approach reveals temperature adaptation of four Nepenthes species.Scientific reports · 2020Article
- Structural Evolution of the Glacier Ice Worm FThe protein journal · 2020Article
- Genomic Insights ofFrontiers in microbiology · 2020Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies of cold-active enzymes have provided basic information on the molecular and biochemical properties of psychrophiles; however, the physiological strategies that compensate for low-temperature metabolism remain poorly understood. We investigated the cellular pools of ATP and ADP in Psychrobacter cryohalolentis K5 incubated at eight temperatures between 22 degrees C and -80 degrees C. Cellular ATP and ADP concentrations increased with decreasing temperature, and the most significant increases were observed in cells that were incubated as frozen suspensions (<-5 degrees C). Respiratory uncoupling significantly decreased this temperature-dependent response, indicating that the proton motive force was required for energy adaptation to frozen conditions. Since ATP and ADP are key substrates in metabolic and energy conservation reactions, increasing their concentrations may provide a strategy for offsetting the kinetic temperature effect, thereby maintaining reaction rates at low temperature. The adenylate levels increased significantly <1 h after freezing and also when the cells were osmotically shocked to simulate the elevated solute concentrations encountered in the liquid fraction of the ice. Together, these data demonstrate that a substantial change in cellular energy metabolism is required for the cell to adapt to the low temperature and water activity conditions encountered during freezing. This physiological response may represent a critical biochemical compensation mechanism at low temperature, have relevance to cellular survival during freezing, and be important for the persistence of microorganisms in icy environments.
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