ArticleJournal of bacteriology2009
Delineation of the species Haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
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- Comparative Genomic Analysis Reveals Genetic Diversity and Pathogenic Potential of Haemophilus seminalis and Emended Description of Haemophilus seminalis.Microbiology spectrum · 2023Article
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- Comparative genomic analysis identifies X-factor (haemin)-independentMicrobial genomics · 2020Article
- Identification and Characterization of "Journal of clinical microbiology · 2019Article
- Complete Whole-Genome Sequence of Haemophilus haemolyticus NCTC 10839.Microbiology resource announcements · 2019Article
- Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan.BMC research notes · 2018Article
- Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination.Journal of clinical microbiology · 2016Article
- Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains.Journal of clinical microbiology · 2016Article
- Complete Deletion of the Fucose Operon in Haemophilus influenzae Is Associated with a Cluster in Multilocus Sequence Analysis-Based Phylogenetic Group II Related to Haemophilus haemolyticus: Implications for Identification and Typing.Journal of clinical microbiology · 2015Article
- Haemophilus influenzae: using comparative genomics to accurately identify a highly recombinogenic human pathogen.BMC genomics · 2015Article
- Difficult identification of Haemophilus influenzae, a typical cause of upper respiratory tract infections, in the microbiological diagnostic routine.European journal of microbiology & immunology · 2015Review
- Non typable-Haemophilus influenzae biofilm formation and acute otitis media.BMC infectious diseases · 2014Article
- Difficulties in species identification within the genus Haemophilus - A pilot study addressing a significant problem for routine diagnostics.European journal of microbiology & immunology · 2014Article
- Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans.Clinical microbiology reviews · 2014Review
- Comparative genomic analysis reveals distinct genotypic features of the emerging pathogen Haemophilus influenzae type f.BMC genomics · 2014Article
- Molecular tools for differentiation of non-typeable Haemophilus influenzae from Haemophilus haemolyticus.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To obtain more information on the much-debated definition of prokaryotic species, we investigated the borders of Haemophilus influenzae by comparative analysis of H. influenzae reference strains with closely related bacteria including strains assigned to Haemophilus haemolyticus, cryptic genospecies biotype IV, and the never formally validated species "Haemophilus intermedius". Multilocus sequence phylogeny based on six housekeeping genes separated a cluster encompassing the type and the reference strains of H. influenzae from 31 more distantly related strains. Comparison of 16S rRNA gene sequences supported this delineation but was obscured by a conspicuously high number of polymorphic sites in many of the strains that did not belong to the core group of H. influenzae strains. The division was corroborated by the differential presence of genes encoding H. influenzae adhesion and penetration protein, fuculokinase, and Cu,Zn-superoxide dismutase, whereas immunoglobulin A1 protease activity or the presence of the iga gene was of limited discriminatory value. The existence of porphyrin-synthesizing strains ("H. intermedius") closely related to H. influenzae was confirmed. Several chromosomally encoded hemin biosynthesis genes were identified, and sequence analysis showed these genes to represent an ancestral genotype rather than recent transfers from, e.g., Haemophilus parainfluenzae. Strains previously assigned to H. haemolyticus formed several separate lineages within a distinct but deeply branching cluster, intermingled with strains of "H. intermedius" and cryptic genospecies biotype IV. Although H. influenzae is phenotypically more homogenous than some other Haemophilus species, the genetic diversity and multicluster structure of strains traditionally associated with H. influenzae make it difficult to define the natural borders of that species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.