ArticleThe Plant cell2008
Arabidopsis Tic40 expression in tobacco chloroplasts results in massive proliferation of the inner envelope membrane and upregulation of associated proteins.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
- A Direct ALAD-SSUII Interaction Implies a Potential Link Between Tetrapyrrole and Terpenoid Pathways Toward Chlorophyll Biosynthesis in Plants.International journal of molecular sciences · 2026Article
- Towards the Albino Mutant Gene inPlants (Basel, Switzerland) · 2024Article
- Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts.The Plant cell · 2024Article
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Plastid Transformation: New Challenges in the Circular Economy Era.International journal of molecular sciences · 2022Review
- Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?Molecules (Basel, Switzerland) · 2022Review
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- MaizeThe Journal of cell biology · 2019Article
- Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules.Frontiers in plant science · 2018Article
- Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure.Scientific reports · 2016Article
- Terpene metabolic engineering via nuclear or chloroplast genomes profoundly and globally impacts off-target pathways through metabolite signalling.Plant biotechnology journal · 2016Article
- Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation.Plant biotechnology journal · 2016Article
- Specific and Efficient Targeting of Cyanobacterial Bicarbonate Transporters to the Inner Envelope Membrane of Chloroplasts in Arabidopsis.Frontiers in plant science · 2016Article
- The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana.PloS one · 2016Article
- The Engineered Chloroplast Genome Just Got Smarter.Trends in plant science · 2015Review
- Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species.Plant biotechnology journal · 2014Article
- Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts.Journal of experimental botany · 2014Article
- How do plants make mitochondria?Planta · 2013Review
- Release of proteins from intact chloroplasts induced by reactive oxygen species during biotic and abiotic stress.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The chloroplast inner envelope membrane (IM) plays essential roles in lipid synthesis, metabolite transport, and cellular signaling in plants. We have targeted a model nucleus-encoded IM protein from Arabidopsis thaliana, pre-Tic40-His, by relocating its expression from the nucleus to the chloroplast genome. Pre-Tic40-His was properly targeted, processed, and inserted. It attained correct topology and was folded and assembled into a TIC complex, where it accounted for up to 15% of the total chloroplast protein. These results confirm the existence of a novel pathway for protein targeting to the IM. Tic40-His overexpression resulted in a massive proliferation of the IM (up to 19 layers in electron micrographs) without significant effects on plant growth or reproduction. Consistent with IM proliferation, the expression levels of other endogenous IM proteins (IEP37, PPT, Tic110) were significantly (10-fold) upregulated but those of outer envelope membrane (Toc159), stromal (hsp93, cpn60), or thylakoid (LHCP, OE23) proteins were not increased, suggesting retrograde signal transduction between chloroplast and nuclear genomes to increase lipid and protein components for accommodation of increased accumulation of Tic40. This study opens the door for understanding the regulation of membrane biogenesis within the organelle and the utilization of transgenic chloroplasts as bioreactors for hyperaccumulation of membrane proteins for biotechnological applications.
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