ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2009
Intervertebral disc cell response to dynamic compression is age and frequency dependent.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Mechanical loading of the intervertebral disc: from the macroscopic to the cellular level.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2014Pooled it
- The effectiveness of walking as an intervention for low back pain: a systematic review.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2010Pooled it
- Preconditioning Mesenchymal Stem Cells to Enhance Performance in the Degenerate Nucleus Pulposus.Tissue engineering. Part B, Reviews · 2026Review
- TRPV4 differentially controls inflammatory cytokine networks during static and dynamic compression of the intervertebral disc.JOR spine · 2023Article
- Blocking toll-like receptor 4 mitigates static loading induced pro-inflammatory expression in intervertebral disc motion segments.Journal of biomechanics · 2023Article
- An in vitro 3D annulus fibrosus cell culture model with type I collagen: An examination of cell-matrix interactions.JOR spine · 2022Article
- TRPV4 mediates cell damage induced by hyperphysiological compression and regulates COX2/PGE2 in intervertebral discs.JOR spine · 2021Article
- Article
- The strain-generated electrical potential in cartilaginous tissues: a role for piezoelectricity.Biophysical reviews · 2021Review
- Intervertebral Disc-on-a-Chip as AdvancedFrontiers in bioengineering and biotechnology · 2021Review
- Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress.Neurospine · 2020Article
- Molecular and histological characteristics of bovine caudal nucleus pulposus by combined changes in hydrostatic and osmotic pressures in vitro.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Article
- Dynamic Hydrostatic Pressure Regulates Nucleus Pulposus Phenotypic Expression and Metabolism in a Cell Density-Dependent Manner.Journal of biomechanical engineering · 2018Article
- Long-term load duration induces N-cadherin down-regulation and loss of cell phenotype of nucleus pulposus cells in a disc bioreactor culture.Bioscience reports · 2017Article
- Tissue Engineering of the Intervertebral Disc's Annulus Fibrosus: A Scaffold-Based Review Study.Tissue engineering and regenerative medicine · 2017Review
- Embryonically inspired scaffolds regulate tenogenically differentiating cells.Journal of biomechanics · 2016Article
- Molecular mechanisms of biological aging in intervertebral discs.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- NF-κB Signaling Pathway in Controlling Intervertebral Disk Cell Response to Inflammatory and Mechanical Stressors.Physical therapy · 2016Article
- Fluid-induced, shear stress-regulated extracellular matrix and matrix metalloproteinase genes expression on human annulus fibrosus cells.Stem cell research & therapy · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The maintenance of the intervertebral disc extracellular matrix is regulated by mechanical loading, nutrition, and the accumulation of matrix proteins and cytokines that are affected by both aging and degeneration. Evidence suggests that cellular aging may lead to alterations in the quantity and quality of extracellular matrix produced. The aims of this study were to examine the role of loading and maturation (a subset of aging), and the interaction between these two factors in intervertebral disc cell gene expression and biosynthesis in a controlled 3D culture environment. Cells were isolated from young (4-6 months) and mature (18-24 months) bovine caudal annulus fibrosus and nucleus pulposus tissue. Isolated cells were seeded into alginate and dynamically compressed for 7 days at either 0.1, 1, or 3 Hz or maintained as a free-swelling control. After 7 days, DNA and sulfated glycosaminoglycan contents were analyzed along with real time, quantitative reverse transcription-polymerase chain reaction analysis for collagen types I and II, aggrecan, and matrix metalloproteinase-3 gene expression. Results suggest that maturation plays an important role in intervertebral disc homeostasis and influences the cell response to mechanical loading. While isolated intervertebral disc cells responded to mechanical compression in 3D culture, the effect of loading frequency was minimal. Altered cellular phenotype and biosynthesis rates appear to be an attribute of the cell maturation process, potentially independent of changes in cellular microenvironment associated with lost nutrition and disc degeneration. Mature cells may have a decreased capacity to create or retain extracellular matrix components in response to mechanical loading compared to young cells.
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