ArticleProceedings of the National Academy of Sciences of the United States of America1991
Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 59 citations in OpenAlex.
- Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.Frontiers in synaptic neuroscience · 2015Article
- The recombinant 5-HT1A receptor: G protein coupling and signalling pathways.British journal of pharmacology · 1999Review
- RGS proteins reconstitute the rapid gating kinetics of gbetagamma-activated inwardly rectifying K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Activation of heteromeric G protein-gated inward rectifier K+ channels overexpressed by adenovirus gene transfer inhibits the excitability of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Receptor stimulation causes slow inhibition of IRK1 inwardly rectifying K+ channels by direct protein kinase A-mediated phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- A G protein-gated K channel is activated via beta 2-adrenergic receptors and G beta gamma subunits in Xenopus oocytes.The Journal of general physiology · 1995Article
- Atrial G protein-activated K+ channel: expression cloning and molecular properties.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Unitary properties of potassium channels activated by 5-HT in acutely isolated rat dorsal raphe neurones.The Journal of physiology · 1993Article
- Slow and incomplete inactivations of voltage-gated channels dominate encoding in synthetic neurons.Biophysical journal · 1993Article
- Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Activation of 5-HT1A receptors expressed in NIH-3T3 cells induces focus formation and potentiates EGF effect on DNA synthesis.Molecular biology of the cell · 1992Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In cardiac atrial cells, muscarinic acetylcholine receptors activate a K+ current directly via a guanine nucleotide-binding protein (G protein). Serotonin type 1A receptors may activate a similar pathway in hippocampal neurons. To develop a system in which receptor/G protein/K+ channel coupling can be experimentally manipulated, we have used a highly efficient recombinant vaccinia virus vector system to express human serotonin 1A receptors in primary cultures of rat atrial myocytes. The expressed 1A receptors activated the inwardly rectifying K+ conductance that is normally activated by the endogenous muscarinic acetylcholine receptors. Maximal responses to either agonist occluded further activation by the other agonist. The average activation time constants for serotonin were about 5 times slower than for acetylcholine. The data support suggestions that the intracellular signaling pathway from seven-helix receptors to G proteins and directly to ion channels is widespread in excitable cells. After a fraction of the G proteins are activated irreversibly by guanosine 5'-[gamma-thio]triphosphate, subsequent transduction proceeds more efficiently. One possible interpretation is that multiple G-protein molecules are required to activate each channel. Vaccinia virus expression vectors are thus useful for expressing seven-helix receptors in primary cultures of postmitotic cells and have provided a heterologous expression system for the signaling pathway from seven-helix receptors to G proteins and directly to ion channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.