ArticleMethods in molecular biology (Clifton, N.J.)2008
Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.
Article in Methods in molecular biology (Clifton, N.J.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Cells that survive acute murine SARS-CoV-2 infection are detected nearly exclusively in the respiratory tract.The Journal of clinical investigation · 2023Article
- Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications.Viruses · 2023Review
- Saccharomyces cerevisiae as a research tool for RNA-mediated human disease.Wiley interdisciplinary reviews. RNA · 2023Review
- Coronavirus Pandemic-Therapy and Vaccines.Biomedicines · 2020Review
- Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein.Virologica Sinica · 2020Article
- Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae.Advances in virus research · 2020Review
- Minimum Determinants of Transmissible Gastroenteritis Virus Enteric Tropism Are Located in the N-Terminus of Spike Protein.Pathogens (Basel, Switzerland) · 2019Article
- Article
- Mutagenesis of Coronavirus nsp14 Reveals Its Potential Role in Modulation of the Innate Immune Response.Journal of virology · 2016Article
- Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome.Virus research · 2014Article
- Efficient generation of recombinant RNA viruses using targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA.BMC genomics · 2013Article
- Reverse genetics of SARS-related coronavirus using vaccinia virus-based recombination.PloS one · 2012Article
- Cloning whole bacterial genomes in yeast.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The construction of coronavirus (CoV) infectious clones had been hampered by the large size of the viral genome (around 30kb) and the instability of plasmids carrying CoV replicase sequences in Escherichia coli. Several approaches have been developed to overcome these problems. Here we describe the engineering of CoV full-length cDNA clones using bacterial artificial chromosomes (BACs). In this system the viral RNA is expressed in the cell nucleus under the control of the cytomegalovirus promoter and further amplified in the cytoplasm by the viral replicase. The BAC-based strategy is an efficient system that allows easy manipulation of CoV genomes to study fundamental viral processes and also to develop genetically defined vaccines. The procedure is illustrated by the cloning of the genome of SARS coronavirus, Urbani strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.