ArticlePLoS genetics2008
Spt2p defines a new transcription-dependent gross chromosomal rearrangement pathway.
Article in PLoS genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- DNA polymerase I and DnaE mediate RNA-templated DNA synthesis over embedded ribonucleotides.Nucleic acids research · 2026Article
- DNA polymerase I is an efficient reverse transcriptase that mediates RNA-templated DNA repair synthesis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Pathways and Mechanisms that Prevent Genome Instability inGenetics · 2017Review
- R-loop: an emerging regulator of chromatin dynamics.Acta biochimica et biophysica Sinica · 2016Review
- Increased Spontaneous Recombination in RNase H2-Deficient Cells Arises From Multiple Contiguous rNMPs and Not From Single rNMP Residues Incorporated by DNA Polymerase Epsilon.Microbial cell (Graz, Austria) · 2016Article
- HMGB1 in health and disease.Molecular aspects of medicine · 2014Review
- Out of balance: R-loops in human disease.PLoS genetics · 2014Review
- Mechanisms of genome instability induced by RNA-processing defects.Trends in genetics : TIG · 2014Review
- Genome-wide profiling of yeast DNA:RNA hybrid prone sites with DRIP-chip.PLoS genetics · 2014Article
- Casein kinase 2 associates with the yeast chromatin reassembly factor Spt2/Sin1 to regulate its function in the repression of spurious transcription.Molecular and cellular biology · 2013Article
- Article
- Transcription as a source of genome instability.Nature reviews. Genetics · 2012Review
- R-loop-mediated genome instability in mRNA cleavage and polyadenylation mutants.Genes & development · 2012Article
- Review
- Transcription regulation by the noncoding RNA SRG1 requires Spt2-dependent chromatin deposition in the wake of RNA polymerase II.Molecular and cellular biology · 2011Article
- The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.Molecular and cellular biology · 2009Article
- Stabilization of dicentric translocations through secondary rearrangements mediated by multiple mechanisms in S. cerevisiae.PloS one · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Large numbers of gross chromosomal rearrangements (GCRs) are frequently observed in many cancers. High mobility group 1 (HMG1) protein is a non-histone DNA-binding protein and is highly expressed in different types of tumors. The high expression of HMG1 could alter DNA structure resulting in GCRs. Spt2p is a non-histone DNA binding protein in Saccharomyces cerevisiae and shares homology with mammalian HMG1 protein. We found that Spt2p overexpression enhances GCRs dependent on proteins for transcription elongation and polyadenylation. Excess Spt2p increases the number of cells in S phase and the amount of single-stranded DNA (ssDNA) that might be susceptible to cause DNA damage and GCR. Consistently, RNase H expression, which reduces levels of ssDNA, decreased GCRs in cells expressing high level of Spt2p. Lastly, high transcription in the chromosome V, the location at which GCR is monitored, also enhanced GCR formation. We propose a new pathway for GCR where DNA intermediates formed during transcription can lead to genomic instability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.