ArticleJournal of structural biology2009
Chromatin position in human HepG2 cells: although being non-random, significantly changed in daughter cells.
Article in Journal of structural biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Dynamic chromosome association with nuclear organelles in living cells.Histochemistry and cell biology · 2024Article
- Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction.GigaScience · 2019Article
- Three-dimensional positioning and structure of chromosomes in a human prophase nucleus.Science advances · 2017Article
- Article
- Non-Random Patterns in the Distribution of NOR-Bearing Chromosome Territories in Human Fibroblasts: A Network Model of Interactions.Journal of cellular physiology · 2015Article
- Functional implications of genome topology.Nature structural & molecular biology · 2013Review
- Something silent this way forms: the functional organization of the repressive nuclear compartment.Annual review of cell and developmental biology · 2013Review
- High-resolution whole-genome sequencing reveals that specific chromatin domains from most human chromosomes associate with nucleoli.Molecular biology of the cell · 2010Article
- Organization of transcription.Cold Spring Harbor perspectives in biology · 2010Review
- Activation of estrogen-responsive genes does not require their nuclear co-localization.PLoS genetics · 2010Article
- Chromosome territories.Cold Spring Harbor perspectives in biology · 2010Review
- Nucleolar tethering mediates pairing between the IgH and Myc loci.Nucleus (Austin, Tex.)Article
- 4D chromatin dynamics in cycling cells: Theodor Boveri's hypotheses revisited.Nucleus (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Mammalian chromosomes occupy chromosome territories within nuclear space the positions of which are generally accepted as non-random. However, it is still controversial whether position of chromosome territories/chromatin is maintained in daughter cells. We addressed this issue and investigated maintenance of various chromatin regions of unknown composition as well as nucleolus-associated chromatin, a significant part of which is composed of nucleolus organizer region-bearing chromosomes. The photoconvertible histone H4-Dendra2 was used to label such regions in transfected HepG2 cells, and its position was followed up to next interphase. The distribution of labeled chromatin in daughter cells exhibited a non-random character. However, its distribution in a vast majority of daughter cells extensively differed from the original ones and the labeled nucleolus-associated chromatin differently located into the vicinity of different nucleoli. Therefore, our results were not consistent with a concept of preservation chromatin position. This conclusion was supported by the finding that the numbers of nucleoli significantly differed between the two daughter cells. Our results support a view that while the transfected daughter HepG2 cells maintain some features of the parental cell chromosome organization, there is also a significant stochastic component associated with reassortment of chromosome territories/chromatin that results in their positional rearrangements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.