ArticleJournal of neuroscience methods2009
Versatile, high-resolution anterograde labeling of vagal efferent projections with dextran amines.
Article in Journal of neuroscience methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Anatomy and function of efferent and afferent vagal innervation of the stomach.Autonomic neuroscience : basic & clinical · 2026Review
- Gut-brain communication: types of sensory nerves and mechanisms of activation.Nature reviews. Gastroenterology & hepatology · 2026Review
- Limited evidence for anatomical contacts between intestinal GLP-1 cells and vagal neurons in male mice.Scientific reports · 2024Article
- Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing.Scientific reports · 2023Article
- Vagal preganglionic axons arborize in the myenteric plexus into two types: nitrergic and non-nitrergic postganglionic motor pools?American journal of physiology. Regulatory, integrative and comparative physiology · 2023Article
- Short-pulsed micro-magnetic stimulation of the vagus nerve.Frontiers in physiology · 2022Article
- Review
- Is Parkinson's disease a chronic low-grade inflammatory bowel disease?Journal of neurology · 2020Review
- Vagal innervation of the stomach reassessed: brain-gut connectome uses smart terminals.Annals of the New York Academy of Sciences · 2019Review
- Avoiding off-target effects in electrical stimulation of the cervical vagus nerve: Neuroanatomical tracing techniques to study fascicular anatomy of the vagus nerve.Journal of neuroscience methods · 2019Review
- Brain-to-stomach transfer of α-synuclein via vagal preganglionic projections.Acta neuropathologica · 2017Article
- Individual sympathetic postganglionic neurons coinnervate myenteric ganglia and smooth muscle layers in the gastrointestinal tract of the rat.The Journal of comparative neurology · 2016Article
- Cellular Organization of Neuroimmune Interactions in the Gastrointestinal Tract.Trends in immunology · 2016Review
- Vagal Intramuscular Arrays: The Specialized Mechanoreceptor Arbors That Innervate the Smooth Muscle Layers of the Stomach Examined in the Rat.The Journal of comparative neurology · 2016Article
- Neural reflex pathways in intestinal inflammation: hypotheses to viable therapy.Nature reviews. Gastroenterology & hepatology · 2015Review
- Central nervous system control of gastrointestinal motility and secretion and modulation of gastrointestinal functions.Comprehensive Physiology · 2014Review
- Gut feelings about smoking and coffee in Parkinson's disease.Movement disorders : official journal of the Movement Disorder Society · 2014Article
- Organization of vagal afferents in pylorus: mechanoreceptors arrayed for high sensitivity and fine spatial resolution?Autonomic neuroscience : basic & clinical · 2014Article
- Neuro-anatomical evidence indicating indirect modulation of macrophages by vagal efferents in the intestine but not in the spleen.PloS one · 2014Article
- Alpha-synuclein expression patterns in the colonic submucosal plexus of the aging Fischer 344 rat: implications for biopsies in aging and neurodegenerative disorders?Neurogastroenterology and motility · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
None of the anterograde tracers used to label and investigate vagal preganglionic neurons projecting to the viscera has proved optimal for routine and extensive labeling of autonomic terminal fields. To identify an alternative tracer protocol, the present experiment evaluated whether dextran conjugates, which have produced superior results in the CNS, might yield widespread and effective labeling of long, fine-caliber vagal efferents in the peripheral nervous system. The dextran conjugates that were evaluated proved reliable and versatile for labeling the motor neuron pool in its entirety, for single- and multiple-labeling protocols, for both conventional and confocal fluorescence microscopy, and for permanent labeling protocols for brightfield microscopy of the projections to the gastrointestinal (GI) tract. Using a standard ABC kit followed by visualization with DAB as the chromagen, Golgi-like labeling of the vagal efferent terminal fields in the GI wall was achieved with the biotinylated dextrans. The definition of individual terminal varicosities was so sharp and detailed that it was routinely practical to examine the relationship of putative vagal efferent contacts (by the criteria of high magnification light microscopy) with the dendritic and somatic architecture of counterstained neurons in the myenteric plexus. Overall, dextran conjugates provide high-definition labeling of an extensive vagal motor pool in the GI tract, and offer considerable versatility when multiple-staining protocols are needed to elucidate the complexities of the innervation of the gut.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.