ArticleBMC genomics2008
Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 52 citations in OpenAlex.
- DNA-Based Tools to Certify Authenticity of Rice Varieties-An Overview.Foods (Basel, Switzerland) · 2022Review
- GMO Genetic Elements Thesaurus (GMO-GET): a controlled vocabulary for the consensus designation of introduced or modified genetic elements in genetically modified organisms.BMC bioinformatics · 2021Article
- Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review.Frontiers in plant science · 2017Review
- Rapid screening of roundup ready soybean in food samples by a hand-held PCR device.Food science and biotechnology · 2016Article
- Current and new approaches in GMO detection: challenges and solutions.BioMed research international · 2015Review
- Comparison and transfer testing of multiplex ligation detection methods for GM plants.BMC biotechnology · 2012Article
- Development of a multiplex DNA-based traceability tool for crop plant materials.Analytical and bioanalytical chemistry · 2012Article
- Molecular techniques for pathogen identification and fungus detection in the environment.IMA fungus · 2011Article
- Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms.Analytical and bioanalytical chemistry · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a limited extent for EU-non-authorised GMOs (NAGs). In the last decade the diversity of genetically modified (GM) ingredients in food and feed has increased significantly. As a result of this increase GMO laboratories currently need to apply many different methods to establish to potential presence of NAGs in raw materials and complex derived products.
resultsIn this paper we present an innovative method for detecting (approved) GMOs as well as the potential presence of NAGs in complex DNA samples containing different crop species. An optimised protocol has been developed for padlock probe ligation in combination with microarray detection (PPLMD) that can easily be scaled up. Linear padlock probes targeted against GMO-events, -elements and -species have been developed that can hybridise to their genomic target DNA and are visualised using microarray hybridisation.In a tenplex PPLMD experiment, different genomic targets in Roundup-Ready soya, MON1445 cotton and Bt176 maize were detected down to at least 1%. In single experiments, the targets were detected down to 0.1%, i.e. comparable to standard qPCR.
conclusionCompared to currently available methods this is a significant step forward towards multiplex detection in complex raw materials and derived products. It is shown that the PPLMD approach is suitable for large-scale detection of GMOs in real-life samples and provides the possibility to detect and/or identify NAGs that would otherwise remain undetected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.