ArticleBMC microbiology2008
Global transcriptional responses of Pseudomonas syringae DC3000 to changes in iron bioavailability in vitro.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 57 citations in OpenAlex.
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- Genome Mining Shows Ubiquitous Presence and Extensive Diversity of Toxin-Antitoxin Systems inFrontiers in microbiology · 2021Article
- Global reprogramming of virulence and antibiotic resistance inThe Journal of biological chemistry · 2020Article
- Multidimensional gene regulatory landscape of a bacterial pathogen in plants.Nature plants · 2020Article
- Signal transduction schemes inComputational and structural biotechnology journal · 2020Review
- Data-Independent Acquisition Proteomics Unravels the Effects of Iron Ions on Coronatine Synthesis inFrontiers in microbiology · 2020Article
- Prevention of Surface-Associated Calcium Phosphate by the Pseudomonas syringae Two-Component System CvsSR.Journal of bacteriology · 2019Article
- Transcriptome landscape of a bacterial pathogen under plant immunity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- CaJournal of bacteriology · 2018Article
- Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola.Plant, cell & environment · 2016Article
- Activity of Gallium Meso- and Protoporphyrin IX against Biofilms of Multidrug-Resistant Acinetobacter baumannii Isolates.Pharmaceuticals (Basel, Switzerland) · 2016Article
- Bypasses in intracellular glucose metabolism in iron-limited Pseudomonas putida.MicrobiologyOpen · 2016Article
- Article
- Transcriptional profile of P. syringae pv. phaseolicola NPS3121 at low temperature: physiology of phytopathogenic bacteria.BMC microbiology · 2013Article
- Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000.Microbiology (Reading, England) · 2013Article
- Pseudomonas syringae pv. tomato DC3000 CmaL (PSPTO4723), a DUF1330 family member, is needed to produce L-allo-isoleucine, a precursor for the phytotoxin coronatine.Journal of bacteriology · 2013Article
- Regulons of three Pseudomonas syringae pv. tomato DC3000 iron starvation sigma factors.Applied and environmental microbiology · 2013Article
- The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.PloS one · 2012Article
- Characterization of the Fur regulon in Pseudomonas syringae pv. tomato DC3000.Journal of bacteriology · 2011Article
- The phytopathogen Pseudomonas syringae pv. tomato DC3000 has three high-affinity iron-scavenging systems functional under iron limitation conditions but dispensable for pathogenesis.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas syringae pv tomato DC3000 (DC3000) is a Gram-negative model plant pathogen that is found in a wide variety of environments. To survive in these diverse conditions it must sense and respond to various environmental cues. One micronutrient required for most forms of life is iron. Bioavailable iron has been shown to be an important global regulator for many bacteria where it not only regulates a wide variety of genes involved in general cell physiology but also virulence determinants. In this study we used microarrays to study differential gene regulation in DC3000 in response to changes in levels of cell-associated iron.
resultsDC3000 cultures were grown under highly controlled conditions and analyzed after the addition of iron citrate or sodium citrate to the media. In the cultures supplemented with iron, we found that cell-associated iron increased rapidly while culture densities were not significantly different over 4 hours when compared to cultures with sodium citrate added. Microarray analysis of samples taken from before and after the addition of either sodium citrate or iron citrate identified 386 differentially regulated genes with high statistical confidence. Differentially regulated genes were clustered based on expression patterns observed between comparison of samples taken at different time points and with different supplements. This analysis grouped genes associated with the same regulatory motifs and/or had similar putative or known function.
conclusionThis study shows iron is rapidly taken up from the medium by iron-depleted DC3000 cultures and that bioavailable iron is a global cue for the expression of iron transport, storage, and known virulence factors in DC3000. Furthermore approximately 34% of the differentially regulated genes are associated with one of four regulatory motifs for Fur, PvdS, HrpL, or RpoD.
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