ArticleBMC genomics2008
A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 97 citations in OpenAlex.
- Stress contingent changes in Hog1 pathway architecture and regulation in Candida albicans.PLoS pathogens · 2024Article
- Histone-like transcription factor Hfl1p inVirulence · 2024Article
- A glycosylated Phr1 protein is induced by calcium stress and its expression is positively controlled by the calcium/calcineurin signaling transcription factor Crz1 in Candida albicans.Cell communication and signaling : CCS · 2023Article
- The proteasome regulator Rpn4 controls antifungal drug tolerance by coupling protein homeostasis with metabolic responses to drug stress.PLoS pathogens · 2023Article
- Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme inLife science alliance · 2022Article
- Transcriptional Profiling of theInternational journal of molecular sciences · 2022Article
- The zinc cluster transcription factor Rha1 is a positive filamentation regulator in Candida albicans.Genetics · 2022Article
- Hsp90 interaction networks in fungi-tools and techniques.FEMS yeast research · 2021Review
- N-acetylglucosamine Signaling: Transcriptional Dynamics of a Novel Sugar Sensing Cascade in a Model Pathogenic Yeast,Journal of fungi (Basel, Switzerland) · 2021Review
- Transcriptional Control of Hypoxic Hyphal Growth in the Fungal PathogenFrontiers in cellular and infection microbiology · 2021Article
- High-Resolution Genome-Wide Occupancy inmSphere · 2020Article
- Article
- Overcoming Fungal Echinocandin Resistance through Inhibition of the Non-essential Stress Kinase Yck2.Cell chemical biology · 2020Article
- Ifu5, a WW domain-containing protein interacts with Efg1 to achieve coordination of normoxic and hypoxic functions to influence pathogenicity traits in Candida albicans.Cellular microbiology · 2020Article
- Characterization of a novel separase-interacting protein and candidate new securin, Eip1p, in the fungal pathogenMolecular biology of the cell · 2019Article
- Article
- The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans.PLoS genetics · 2019Article
- Mms21: A Putative SUMO E3 Ligase inGenetics · 2019Article
- Integration of Growth and Cell Size via the TOR Pathway and the Dot6 Transcription Factor inGenetics · 2019Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCandida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing microbial agent.
resultsWe have developed a toolbox allowing in vivo protein tagging by PCR-mediated homologous recombination with TAP, HA and MYC tags. The transformation cassettes were designed to accommodate a common set of integration primers. The tagged proteins can be used to perform tandem affinity purification (TAP) or chromatin immunoprecipitation coupled with microarray analysis (ChIP-CHIP). Tandem affinity purification of C. albicans Nop1 revealed the high conservation of the small processome composition in yeasts. Data obtained with in vivo TAP-tagged Tbf1, Cbf1 and Mcm1 recapitulates previously published genome-wide location profiling by ChIP-CHIP. We also designed a new reporter system for in vivo analysis of transcriptional activity of gene loci in C. albicans.
conclusionThis toolbox provides a basic setup to perform purification of protein complexes and increase the number of annotated transcriptional regulators and genetic circuits in C. albicans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.