ArticleThe Journal of biological chemistry2009
Membrane hyperpolarization drives cation influx and fungicidal activity of amiodarone.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 77 citations in OpenAlex.
- Anandamide Targets Membrane Integrity in Non-Journal of fungi (Basel, Switzerland) · 2026Article
- Trk1 potassium transport is crucial for effectiveProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Marine-Derived Metabolites Act as Promising Antifungal Agents.Marine drugs · 2024Review
- Exit, O Sodium!Function (Oxford, England) · 2024Article
- Calcium Ion Channels inJournal of fungi (Basel, Switzerland) · 2023Review
- Splicing factor SRSF3 represses translation of p21Cell death & disease · 2022Article
- Aequorin as a Useful Calcium-Sensing Reporter inJournal of fungi (Basel, Switzerland) · 2021Article
- Emerging Roles of SRSF3 as a Therapeutic Target for Cancer.Frontiers in oncology · 2020Review
- Article
- Plasma Membrane Potential ofMicroorganisms · 2019Article
- Chemogenomic profiling in yeast reveals antifungal mode-of-action of polyene macrolactam auroramycin.PloS one · 2019Article
- Hyper-Osmotic Stress Elicits Membrane Depolarization and Decreased Permeability in Halotolerant MarineFrontiers in microbiology · 2019Article
- Amiodarone promotes cancer cell death through elevated truncated SRSF3 and downregulation of miR-224.Oncotarget · 2018Article
- Acridine yellow. A novel use to estimate and measure the plasma membrane potential in Saccharomyces cerevisiae.Journal of bioenergetics and biomembranes · 2017Article
- Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons.Journal of fluorescence · 2014Article
- Article
- Attenuation of Candida albicans virulence with focus on disruption of its vacuole functions.Journal of oral microbiology · 2014Review
- Early changes in membrane potential of Saccharomyces cerevisiae induced by varying extracellular K(+), Na (+) or H (+) concentrations.Journal of bioenergetics and biomembranes · 2013Article
- Synergistic effects of amiodarone and fluconazole on Candida tropicalis resistant to fluconazole.Antimicrobial agents and chemotherapy · 2013Article
- An integrative model of ion regulation in yeast.PLoS computational biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cationic amphipathic drugs, such as amiodarone, interact preferentially with lipid membranes to exert their biological effect. In the yeast Saccharomyces cerevisiae, toxic levels of amiodarone trigger a rapid influx of Ca(2+) that can overwhelm cellular homeostasis and lead to cell death. To better understand the mechanistic basis of antifungal activity, we assessed the effect of the drug on membrane potential. We show that low concentrations of amiodarone (0.1-2 microm) elicit an immediate, dose-dependent hyperpolarization of the membrane. At higher doses (>3 microm), hyperpolarization is transient and is followed by depolarization, coincident with influx of Ca(2+) and H(+) and loss in cell viability. Proton and alkali metal cation transporters play reciprocal roles in membrane polarization, depending on the availability of glucose. Diminishment of membrane potential by glucose removal or addition of salts or in pma1, tok1Delta, ena1-4Delta, or nha1Delta mutants protected against drug toxicity, suggesting that initial hyperpolarization was important in the mechanism of antifungal activity. Furthermore, we show that the link between membrane hyperpolarization and drug toxicity is pH-dependent. We propose the existence of pH- and hyperpolarization-activated Ca(2+) channels in yeast, similar to those described in plant root hair and pollen tubes that are critical for cell elongation and growth. Our findings illustrate how membrane-active compounds can be effective microbicidals and may pave the way to developing membrane-selective agents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.